Brand  (β version)

PDB ID: 2W8S

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Ligands
Code Name Style Show Link
FE Fe (III) ion PoSSuM
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
FGL 2-aminopropanedioic acid
Glycosylation
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Structure summary

Code : 2W8S   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : CRYSTAL STRUCTURE OF A catalytically promiscuous PHOSPHONATE MONOESTER HYDROLASE FROM Burkholderia caryophylli
Release Data : 2010-02-23
Compound :
mol_id molecule chains
1 PHOSPHONATE MONOESTER HYDROLASE A,B,C,D
other_details: AT POSITION 57 OF THE PROTEIN CHAINS A, B, C, D. BOTH CYS AND FGL ARE PRESENT AT 0.80 AND 0.20 OCCUPANCIES, RESPECTIVELY.
Source :
mol_id organism_scientific expression_system
1 BURKHOLDERIA CARYOPHYLLI  (taxid:28094) ESCHERICHIA COLI  (taxid:469008)
strain: BG2952
expression_system_strain: BL21(DE3)
expression_system_plasmid: PASK-IBA5PLUS
Authors : Jonas, S., van Loo, B., Hyvonen, M., Hollfelder, F.
Keywords : HYDROLASE, FORMYLGLYCINE, PHOSPHODIESTERASE, ALKALINE PHOSPHATASE SUPERFAMILY, CATALYTIC PROMISCUITY, PHOSPHONATE MONOESTER HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.40 Å )
Citation :

An Efficient, Multiply Promiscuous Hydrolase in the Alkaline Phosphatase Superfamily.

Van Loo, B.,Jonas, S.,Babtie, A.C.  et al.
(2010)  Proc.Natl.Acad.Sci.USA  107 : 2740

PubMed: 20133613
DOI: 10.1073/PNAS.0903951107

A New Member of the Alkaline Phosphatase Superfamily with a Formylglycine Nucleophile: Structural and Kinetic Characterisation of a Phosphonate Monoester Hydrolase/Phosphodiesterase from Rhizobium Leguminosarum.

Jonas, S.,Van Loo, B.,Hyvonen, M.  et al.
(2008)  J.Mol.Biol.  384 : 120

PubMed: 18793651
DOI: 10.1016/J.JMB.2008.08.072

Reaction

Chain : A, B, C, D
UniProt : Q45087 (BCPMH_TRICW)
Reaction : -