Brand  (β version)

PDB ID: 2W0D

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
CA Calcium ion PoSSuM
ACT Acetate ion PoSSuM
CGS N-hydroxy-2(R)-[[(4-methoxyphenyl)sulfonyl](3-picolyl)amino]-3-methylbutanamide hydrochloride PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 2W0D   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Does a Fast Nuclear Magnetic Resonance Spectroscopy- and X-Ray Crystallography Hybrid Approach Provide Reliable Structural Information of Ligand-Protein Complexes? A Case Study of Metalloproteinases.
Release Data : 2009-03-03
Compound :
mol_id molecule chains synonym
1 MACROPHAGE METALLOELASTASE A,B,C,D MME, MATRIX METALLOPROTEINASE-12, MACROPHAGE ELASTASE, ME, HME, MMP-12
ec: 3.4.24.65
fragment: CATALYTIC DOMAIN, RESIDUES 106-263
mutation: YES
other_details: COMPOUND EXPRESSED WITH A 5XHIS TAG AT THE C-TERMINUS FOR CLONING/PURIFICATION PURPOSES
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: PET11A
Authors : Isaksson, J., Nystrom, S., Derbyshire, D.J., Wallberg, H., Agback, T., Kovacs, H., Bertini, I., Felli, I.C.
Keywords : GLYCOPROTEIN, METAL-BINDING, METALLOPROTEASE, SECRETED, HYDROLASE, HYDROXAMATE, EXTRACELLULAR MATRIX, COPD, MMP-12, ZYMOGEN
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Does a Fast Nuclear Magnetic Resonance Spectroscopy- and X-Ray Crystallography Hybrid Approach Provide Reliable Structural Information of Ligand-Protein Complexes? a Case Study of Metalloproteinases.

Isaksson, J.,Nystrom, S.,Derbyshire, D.J.  et al.
(2009)  J.Med.Chem.  52 : 1712

PubMed: 19239231
DOI: 10.1021/JM801388Q

Reaction

Chain : A, B, C, D
UniProt : P39900 (MMP12_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin. 3.4.24.65 -
-