Brand  (β version)

PDB ID: 2VL8

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Ligands
Code Name Style Show Link
UDP Uridine-5'-diphosphate PoSSuM
CTS Castanospermine PoSSuM
CA Calcium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2VL8   PDBj   RCSB PDB   PDBe
Header : TOXIN
Title : CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF LETHAL TOXIN FROM CLOSTRIDIUM SORDELLII IN COMPLEX WITH UDP, CASTANOSPERMINE AND CALCIUM ION
Release Data : 2008-06-17
Compound :
mol_id molecule chains
1 CYTOTOXIN L A,B,C
fragment: CATALYTIC DOMAIN, RESIDUES 1-546
mutation: YES
Source :
mol_id organism_scientific expression_system
1 CLOSTRIDIUM SORDELLII  (taxid:1505) ESCHERICHIA COLI  (taxid:562)
strain: 6018
Authors : Jank, T., Ziegler, M.O.P., Schulz, G.E., Aktories, K.
Keywords : TOXIN, GLYCOSYLTRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.31 Å )
Citation :

Inhibition of the Glucosyltransferase Activity of Clostridial Rho/Ras-Glucosylating Toxins by Castanospermine.

Jank, T.,Ziegler, M.O.P.,Schulz, G.E.  et al.
(2008)  FEBS Lett.  582 : 2277

PubMed: 18505687
DOI: 10.1016/J.FEBSLET.2008.05.025

Reaction

Chain : A, B, C
UniProt : Q46342 (TCSL1_PARSO)
Reaction: EC: Evidence:
Physiological Direction:
[Glucosyltransferase TcsL]
L-threonyl-[protein] + UDP-alpha-D-glucose = 3-O-(alpha-D- glucosyl)-L-threonyl-[protein] + UDP + H(+) 		- PubMed:17901056, PubMed:19744486, PubMed:24905543, PubMed:24919149, PubMed:27023605, PubMed:27303685, PubMed:30622517, PubMed:8626575, PubMed:8626586, PubMed:8858106, PubMed:9632667
left-to-right
Cofactor: Evidence: Note:
Zn(2+) ECO:0000250 | UniProtKB:P18177
Binds 1 Zn(2+) ion per subunit. Zn(2+) is required for autocatalytic cleavage.
Mg(2+) ECO:0000269 | PubMed:27089365
ECO:0000269 | PubMed:8626575
ECO:0000269 | PubMed:27089365
Has higher activity with Mn(2+), but most likely uses Mg(2+) in host cells (PubMed:27089365). Mn(2+) or Mg(2+) are required for glucosyltransferase activity (PubMed:27089365, PubMed:8626575).