Brand  (β version)

PDB ID: 2VF0

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Ligands
Code Name Style Show Link
F89 S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo(f)quinazolin-9-yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid PoSSuM
NDU 2'-deoxy-5-nitrouridine 5'-monophosphate PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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CXM N-carboxymethionine
Glycosylation
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Structure summary

Code : 2VF0   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : CRYSTAL STRUCTURE OF THE THYMIDYLATE SYNTHASE K48Q COMPLEXED WITH 5NO2DUMP AND BW1843U89
Release Data : 2007-12-04
Compound :
mol_id molecule chains synonym
1 THYMIDYLATE SYNTHASE A,B TS, TSASE,
ec: 2.1.1.45
mutation: YES
other_details: MUTANT K48Q COMPLEXED WITH 5-NO2DUMP AND BW1843U89
Source :
mol_id organism_scientific expression_system
1 ESCHERICHIA COLI  (taxid:511693) ESCHERICHIA COLI  (taxid:562)
strain: BL21
expression_system_vector_type: PLASMID
expression_system_plasmid: PET17B
Authors : Sotelo-Mundo, R.R., Arreola, R., Maley, F., Montfort, W.R.
Keywords : NUCLEOTIDE BIOSYNTHESIS, SUBSTRATE ANOLOGUE, ANTIFOLATE BINDING, RNA-BINDING, TRANSFERASE, METHYLTRANSFERASE, REPRESSOR, BW1843U89, CYTOPLASM, 5-NO2-DUMP, THYMIDYLATE SYNTHASE, TRANSLATION REGULATION
Exp. method : X-RAY DIFFRACTION ( 3.00 Å )
Citation :

Role of an Invariant Lysine Residue in Folate Binding on Escherichia Coli Thymidylate Synthase: Calorimetric and Crystallographic Analysis of the K48Q Mutant.

Arvizu-Flores, A.A.,Sugich-Miranda, R.,Arreola, R.  et al.
(2008)  Int.J.Biochem.Cell Biol.  40 : 2206

PubMed: 18403248
DOI: 10.1016/J.BIOCEL.2008.02.025

Reaction

Chain : A, B
UniProt : P0A884 (TYSY_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP 2.1.1.45 HAMAP-Rule:MF_00008, PubMed:2223754, PubMed:3286637, PubMed:9826509
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