Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
EDO 1,2-ethanediol
Modified Residues
Code Name Link
CJO [(2r,4r)-2-[(1r,2r)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzyl)-5-oxoimidazolidin-1-yl]acetic acid
Code : 2QT2
Header : FLUORESCENT PROTEIN
Title : Cyclized-Dehydrated Intermediate of GFP Variant Q183E in Chromophore Maturation
Release Data : 2009-04-21
Compound :
mol_id molecule chains
1 Green fluorescent protein A
mutation: F64L, F99S, M153T, V163A, Q183E
Source :
mol_id organism_scientific organism_common expression_system
1 Aequorea victoria  (taxid:6100) Jellyfish Escherichia coli  (taxid:562)
gene: GFP
expression_system_strain: BL21/DE3, pRIL
expression_system_vector_type: plasmid
expression_system_plasmid: pTIW188
Authors : Wood, T.I., Barondeau, D.P., Hitomi, C., Tainer, J.A., Getzoff, E.D.
Keywords : GFP, chromophore maturation, cyclized, dehydrated intermediate, Chromophore, Luminescence, Photoprotein, FLUORESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.31 Å )
Citation :

Kinetically Isolated Reaction Intermediates Provide Structural Characterization of the Green Fluorescence Protein Fluorophore Biosynthesis Pathway

Wood, T.I.,Barondeau, D.P.,Hitomi, C.  et al.
To be Published 

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -