Brand  (β version)

PDB ID: 2P80

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Ligands
Code Name Style Show Link
CU Copper (II) ion PoSSuM
GD Gadolinium atom PoSSuM
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Structure summary

Code : 2P80   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Solution structure of the complex between nitrite reductase and pseudoazurin from A. faecalis
Release Data : 2007-12-11
Compound :
mol_id molecule chains synonym
1 Copper-containing nitrite reductase A,B,C Cu-NIR
ec: 1.7.2.1
mol_id molecule chains synonym
2 Pseudoazurin D Cupredoxin, Blue copper protein
Source :
mol_id organism_scientific expression_system
1 Alcaligenes faecalis  (taxid:511) Escherichia coli BL21(DE3)  (taxid:469008)
strain: S-6
gene: nirK, nir
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28A
mol_id organism_scientific expression_system
2 Alcaligenes faecalis  (taxid:511) Escherichia coli BL21(DE3)  (taxid:469008)
strain: S-6
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28A
Authors : Vlasie, M.D., Ubbink, M.
Keywords : transient complex, protein-protein interaction, redox partners, electron transfer, OXIDOREDUCTASE
Exp. method : SOLUTION NMR
Citation :

Low resolution solution structure of the 152 kDa complex between nitrite reductase and pseudoazurin from A. faecalis by paramagnetic NMR.

Vlasie, M.D.,Fernandez-Busnadiego, R.,Prudencio, M.  et al.
To be Published 

Reaction

Chain : A, B, C
UniProt : P38501 (NIR_ALCFA)
Reaction: EC: Evidence:
Physiological Direction:
nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)- [cytochrome c] + nitrite + 2 H(+) 1.7.2.1 -
-
Cofactor: Evidence: Note:
Cu(2+) ECO:0000250
Binds 1 Cu(2+) ion. The Cu(2+) ion is held by residues from each of 2 monomers of the trimer. Nitrite is bound to the Cu(2+) ion site. Pseudoazurin is the physiological electron donor for the Cu-NIR in vitro.
Cu(+) ECO:0000250
Binds 1 Cu(+) ion. The Cu(+) ion is bound within a single monomer.
FAD ECO:0000250
Binds 1 Cu(+) ion. The Cu(+) ion is bound within a single monomer.
Chain : D
UniProt : P04377 (AZUP_ALCFA)
Reaction : -
Cofactor: Evidence: Note:
Cu cation - Binds 1 copper ion per subunit.