Brand  (β version)

PDB ID: 2OX0

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Ligands
Code Name Style Show Link
NI Nickel (II) ion PoSSuM
ZN Zinc ion PoSSuM
CL Chloride ion PoSSuM
OGA N-oxalylglycine PoSSuM
Non-standard Residues
Code Name Show
MLY N-dimethyl-lysine
ALY N(6)-acetyllysine
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 2OX0   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of JMJD2A complexed with histone H3 peptide dimethylated at Lys9
Release Data : 2007-03-13
Compound :
mol_id molecule chains synonym
1 JmjC domain-containing histone demethylation protein 3A A,B Jumonji domain-containing protein 2A
ec: 1.14.11.-
mol_id molecule chains
2 synthetic peptide C,D
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: JMJD2A, JHDM3A, JMJD2, KIAA0677
expression_system_strain: BL21(DE3)-R3
expression_system_vector_type: PLASMID
expression_system_plasmid: PNIC28-BSA4
mol_id organism_scientific
2
synthetic: yes
other_details: synthetic peptide
Authors : Pilka, E.S., Ng, S.S., Kavanagh, K.L., McDonough, M.A., Savitsky, P., von Delft, F., Arrowsmith, C.H., Weigelt, J., Edwards, A., Sundstrom, M., Schofield, C.J., Oppermann, U., Structural Genomics Consortium (SGC)
Keywords : double-stranded beta helix, demethylase, oxygenase, SGC, STRUCTURAL GENOMICS, STRUCTURAL GENOMICS CONSORTIUM, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Crystal structures of histone demethylase JMJD2A reveal basis for substrate specificity.

Ng, S.S.,Kavanagh, K.L.,McDonough, M.A.  et al.
(2007)  Nature  448 : 87 - 91

PubMed: 17589501
DOI: 10.1038/nature05971

Reaction

Chain : A, B
UniProt : O75164 (KDM4A_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
2 2-oxoglutarate + N(6),N(6),N(6)-trimethyl-L-lysyl(9)- [histone H3] + 2 O2 = 2 CO2 + 2 formaldehyde + N(6)-methyl-L- lysyl(9)-[histone H3] + 2 succinate 1.14.11.66 PubMed:16603238
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2 2-oxoglutarate + N(6),N(6),N(6)-trimethyl-L-lysyl(36)- [histone H3] + 2 O2 = 2 CO2 + 2 formaldehyde + N(6)-methyl-L- lysyl(36)-[histone H3] + 2 succinate 1.14.11.69 PubMed:16603238
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