Brand  (β version)

PDB ID: 2OD2

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Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
CNA Carba-nicotinamide-adenine-dinucleotide PoSSuM
GOL Glycerol PoSSuM
Non-standard Residues
Code Name Show
ALY N(6)-acetyllysine
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 2OD2   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal Structure of yHst2 I117F mutant bound to carba-NAD+ and an acetylated H4 peptide
Release Data : 2007-02-20
Compound :
mol_id molecule chains synonym
1 NAD-dependent deacetylase HST2 A Homologous to SIR2 protein 2
ec: 3.5.1.-
fragment: Hst2 catalytic core domain, residues 1-294
mutation: I117F
mol_id molecule chains
2 Acetylated H4 peptide B
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Baker's yeast Escherichia coli  (taxid:562)
gene: HST2
expression_system_strain: DE3 BL-21 gold
expression_system_vector_type: plasmid
expression_system_plasmid: pRSET-A
mol_id organism_scientific
2
synthetic: yes
other_details: This sequence occurs naturally in Homo sapiens (Humans)
Authors : Sanders, B.D.
Keywords : Zn binding protein, Rossmann fold, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.000 Å )
Citation :

Structural basis for nicotinamide inhibition and base exchange in sir2 enzymes.

Sanders, B.D.,Zhao, K.,Slama, J.T.  et al.
(2007)  Mol.Cell  25 : 463 - 472

PubMed: 17289592
DOI: 10.1016/j.molcel.2006.12.022

Reaction

Chain : A
UniProt : P53686 (HST2_YEAST)
Reaction: EC: Evidence:
Physiological Direction:
N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl- ADP-D-ribose + nicotinamide + L-lysyl-[protein] 2.3.1.286 PROSITE-ProRule:PRU00236, PubMed:16520376
-
Cofactor: Evidence: Note:
Zn(2+) - Binds 1 zinc ion per subunit.