Brand  (β version)

PDB ID: 2II5

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Ligands
Code Name Style Show Link
ACT Acetate ion PoSSuM
CL Chloride ion PoSSuM
CO6 Isobutyryl-coenzyme a PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2II5   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of a cubic core of the dihydrolipoamide acyltransferase (E2b) component in the branched-chain alpha-ketoacid dehydrogenase complex (BCKDC), Isobutyryl-Coenzyme A-bound form
Release Data : 2006-12-26
Compound :
mol_id molecule chains synonym
1 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex A,B,C,D,E,F,G,H Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, E2, Dihydrolipoamide branched chain transacylase, BCKAD E2 subunit
ec: 2.3.1.168
fragment: core (catalytic) domain
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Cattle Escherichia coli BL21(DE3)  (taxid:469008)
gene: DBT
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
Authors : Kato, M., Wynn, R.M., Chuang, J.L., Brautigam, C.A., Custorio, M., Chuang, D.T.
Keywords : cubic core, homo trimer, Isobutyryl-CoA-bound form, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.500 Å )
Citation :

A synchronized substrate-gating mechanism revealed by cubic-core structure of the bovine branched-chain alpha-ketoacid dehydrogenase complex.

Kato, M.,Wynn, R.M.,Chuang, J.L.  et al.
(2006)  Embo J.  25 : 5983 - 5994

PubMed: 17124494
DOI: 10.1038/sj.emboj.7601444

Reaction

Chain : A, B, C, D, E, F, G, H
UniProt : P11181 (ODB2_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 2- methylpropanoyl-CoA = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]- L-lysyl-[protein] + CoA 2.3.1.168 PubMed:283398
left-to-right
Cofactor: Evidence: Note:
(R)-lipoate - Binds 1 lipoyl cofactor covalently.