Brand  (β version)

PDB ID: 2I82

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
FOU (5s,6r)-5-fluoro-6-hydroxydihydropyrimidine-2,4(1h,3h)-dione PoSSuM
Non-standard Residues
Code Name Show
MSE Selenomethionine
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 2I82   PDBj   RCSB PDB   PDBe
Header : Lyase/RNA
Title : Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure
Release Data : 2006-11-21
Compound :
mol_id molecule chains
1 5'-R(*GP*AP*GP*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP*CP*UP*C)-3' E,F,G,H
mol_id molecule chains synonym
2 Ribosomal large subunit pseudouridine synthase A A,B,C,D rRNA-uridine lyase A, rRNA pseudouridylate synthase A
ec: 4.2.1.70
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli BL21(DE3)  (taxid:469008)
gene: rluA
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET15-b
Authors : Hoang, C.
Keywords : pseudouridine synthase, Lyase-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.05 Å )
Citation :

Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure

Hoang, C.,Chen, J.,Vizthum, C.A.  et al.
(2006)  Mol.Cell  24 : 535 - 545

PubMed: 17188032
DOI: 10.1016/j.molcel.2006.09.017

Reaction

Chain : A, B, C, D
UniProt : P0AA37 (RLUA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
uridine(32) in tRNA = pseudouridine(32) in tRNA 5.4.99.28 PubMed:7493321
-
uridine(746) in 23S rRNA = pseudouridine(746) in 23S rRNA 5.4.99.29 PubMed:7493321
-