Brand  (β version)

PDB ID: 2HHW

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
SO4 Sulfate ion PoSSuM
D3T 2',3'-dideoxy-thymidine-5'-triphosphate PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
FRU Beta-D-fructofuranose PoSSuM
: Polysaccharide
Non-standard Residues
Code Name Show
DDG 2',3'-dideoxy-guanosine-5'-monophosphate
6OG 6-O-methyl guanosine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 2HHW   PDBj   RCSB PDB   PDBe
Header : Transferase/DNA
Title : ddTTP:O6-methyl-guanine pair in the polymerase active site, in the closed conformation
Release Data : 2006-12-12
Compound :
mol_id molecule chains
1 5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DDG))-3' E,B
other_details: DNA Primer Strand
mol_id molecule chains
2 5'-D(*CP*AP*TP*(6OG)P*CP*GP*AP*GP*TP*CP*AP*GP*G)-3' F,C
other_details: DNA Template Strand
mol_id molecule chains
3 DNA Polymerase I A,D
ec: 2.7.7.7
fragment: residues 299-876 (analogous to E coli Klenow fragment)
mutation: D598A, F710Y
Source :
mol_id organism_scientific
1
synthetic: yes
other_details: chemically synthesized
mol_id organism_scientific
2
synthetic: yes
other_details: chemically synthesized
mol_id organism_scientific expression_system
3 Geobacillus stearothermophilus  (taxid:1422) Escherichia coli BL21  (taxid:511693)
gene: polA
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: PUC
Authors : Warren, J.J., Forsberg, L.J., Beese, L.S.
Keywords : DNA polymerase I, DNA replication, Klenow fragment, DNA-protein complex, O6-methyl-guanine, Transferase-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.880 Å )
Citation :

The structural basis for the mutagenicity of O6-methyl-guanine lesions.

Warren, J.J.,Forsberg, L.J.,Beese, L.S.
(2006)  Proc.Natl.Acad.Sci.Usa  103 : 19701 - 19706

PubMed: 17179038
DOI: 10.1073/pnas.0609580103

Reaction

Chain : A, D
UniProt : Q5KWC1 (Q5KWC1_GEOKA)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 ARBA:ARBA00024632, RuleBase:RU004460
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