Brand  (β version)

PDB ID: 2GVD

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Ligands
Code Name Style Show Link
128 Spiro(2,4,6-trinitrobenzene[1,2a]-2o',3o'-methylene-adenine-triphosphate PoSSuM
CL Chloride ion PoSSuM
FKP Methylpiperazinoforskolin PoSSuM
GSP 5'-guanosine-diphosphate-monothiophosphate PoSSuM
MN Manganese (II) ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2GVD   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Complex Of Gs- With The Catalytic Domains Of Mammalian Adenylyl Cyclase: Complex With TNP-ATP and Mn
Release Data : 2006-07-04
Compound :
mol_id molecule chains synonym
1 Adenylate cyclase type 5 A Adenylate cyclase type V, ATP pyrophosphate-lyase 5, Adenylyl cyclase 5, Ca2+, -inhibitable adenylyl cyclase
ec: 4.6.1.1
fragment: C1A domain, residues 440-657
mol_id molecule chains synonym
2 Adenylate cyclase type 2 B Adenylate cyclase type II, ATP pyrophosphate-lyase 2, Adenylyl cyclase 2
ec: 4.6.1.1
fragment: C2A domain, residues 870-1081
mol_id molecule chains synonym
3 Guanine nucleotide-binding protein G(s), alpha subunit C Adenylate cyclase-stimulating G alpha protein
Source :
mol_id organism_scientific organism_common expression_system
1 Canis lupus familiaris  (taxid:9615) Dog Escherichia coli BL21  (taxid:511693)
strain: familiaris
gene: ADCY5
expression_system_strain: BL21
expression_system_vector_type: PLASMID
expression_system_plasmid: PQE60-H6-VC1(580)
mol_id organism_scientific organism_common expression_system
2 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21  (taxid:511693)
gene: Adcy2
expression_system_strain: BL21
expression_system_vector_type: PLASMID
expression_system_plasmid: PQE60-IIC2
mol_id organism_scientific organism_common expression_system
3 Bos taurus  (taxid:9913) Cattle Escherichia coli BL21  (taxid:511693)
gene: GNAS, GNAS1
expression_system_strain: BL21
expression_system_vector_type: PLASMID
expression_system_plasmid: PQE60-H6-G(S)ALPHA
Authors : Mou, T.-C., Sprang, S.R.
Keywords : ADENYLYL CYCLASE, GSA, TNP-ATP, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.90 Å )
Citation :

Broad specificity of Mammalian adenylyl cyclase for interaction with 2',3'-substituted purine- and pyrimidine nucleotide inhibitors.

Mou, T.-C.,Gille, A.,Suryanarayana, S.  et al.
(2006)  Mol.Pharmacol.  70 : 878 - 886

PubMed: 16766715
DOI: 10.1124/mol.106.026427

Reaction

Chain : A
UniProt : P30803 (ADCY5_CANLF)
Reaction: EC: Evidence:
Physiological Direction:
ATP = 3',5'-cyclic AMP + diphosphate 4.6.1.1 PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:1618857, PubMed:16766715, PubMed:19243146, PubMed:8119955, PubMed:8428899, PubMed:9748257
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
ECO:0000269 | PubMed:19243146
ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
Binds 2 magnesium ions per subunit (PubMed:16766715, PubMed:19243146). Is also active with manganese (in vitro) (PubMed:11087399, PubMed:16766715).
Chain : B
UniProt : P26769 (ADCY2_RAT)
Reaction: EC: Evidence:
Physiological Direction:
ATP = 3',5'-cyclic AMP + diphosphate 4.6.1.1 PubMed:10427002, PubMed:11087399, PubMed:15591060, PubMed:16766715, PubMed:1719547, PubMed:19243146, PubMed:21596131, PubMed:22906005, PubMed:24363043, PubMed:7761832
-
Cofactor: Evidence: Note:
Mn(2+) ECO:0000269 | PubMed:10427002
ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
ECO:0000269 | PubMed:1719547
ECO:0000269 | PubMed:19243146
ECO:0000269 | PubMed:7761832
ECO:0000269 | PubMed:10427002
ECO:0000269 | PubMed:11087399
ECO:0000269 | PubMed:16766715
Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).
Chain : C
UniProt : P04896 (GNAS2_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
GTP + H2O = GDP + phosphate + H(+) - UniProtKB:P63092
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