Brand  (β version)

PDB ID: 2GSK

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
CNC Cyanocobalamin PoSSuM
HEX Hexane PoSSuM
LDA Lauryl dimethylamine-N-oxide PoSSuM
OCT N-octane PoSSuM
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Structure summary

Code : 2GSK   PDBj   RCSB PDB   PDBe
Header : SIGNALING PROTEIN/MEMBRANE PROTEIN
Title : Structure of the BtuB:TonB Complex
Release Data : 2006-06-13
Compound :
mol_id molecule chains synonym
1 Vitamin B12 transporter btuB A BTUB; Cobalamin receptor; Outer membrane cobalamin translocator
mol_id molecule chains
2 protein TONB B
fragment: C-TERMINAL DOMAIN
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: btuB, bfe, cer, dcrC
expression_system_strain: BL21(DE3)PLYSS
expression_system_vector_type: plasmid
expression_system_plasmid: PET22B
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli BL21(DE3)  (taxid:469008)
gene: tonB, exbA
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: PGEX4T1
Authors : Shultis, D.D., Purdy, M.P., Banchs, C.N., Wiener, M.C.
Keywords : OUTER-MEMBRANE ACTIVE TRANSPORT, BETA-BARREL, TONB, MEMBRANE PROTEIN, SIGNALING PROTEIN-MEMBRANE PROTEIN COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Outer membrane active transport: structure of the BtuB:TonB complex

Shultis, D.D.,Purdy, M.D.,Banchs, C.N.  et al.
(2006)  Science  312 : 1396 - 1399

PubMed: 16741124
DOI: 10.1126/science.1127694

Crystallization and preliminary x-ray crystallographic analysis of the Escherichia coli outer membrane cobalamin transporter BtuB in complex with the carboxy-terminal domain of TonB

Shultis, D.D.,Purdy, M.D.,Banchs, C.N.  et al.
(2006)  Acta Crystallogr.,Sect.F 

Reaction

Chain : A
UniProt : P06129 (BTUB_ECOLI)
Reaction : -
Chain : B
UniProt : P94739 (TONB_ECOLI)
Reaction : -