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Ligands
Code Name Style Show Link
ALF Tetrafluoroaluminate ion
MG Magnesium ion
K Potassium ion
SE Selenium atom
GDP Guanosine-5'-diphosphate
Non-standard Residues
Code Name Show
MSE Selenomethionine
Glycosylation
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Modification
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Code : 2GJ8   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Structure of the MnmE G-domain in complex with GDP*AlF4-, Mg2+ and K+
Release Data : 2006-07-04
Compound :
mol_id molecule chains synonym
1 tRNA modification GTPase trmE A,B,C,D mnmE
fragment: G-domain (216-384)
Source :
mol_id organism_scientific expression_system
1 Escherichia coli BL21(DE3)  (taxid:469008) Escherichia coli BL21(DE3)  (taxid:469008)
strain: BL21DE3
gene: trmE, mnmE, thdF
expression_system_strain: BL21DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pET 14b
Authors : Scrima, A., Wittinghofer, A.
Keywords : G-domain dimer, alpha-beta-sandwich, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Dimerisation-dependent GTPase reaction of MnmE: how potassium acts as GTPase-activating element.

Scrima, A.,Wittinghofer, A.
(2006)  Embo J.  25 : 2940 - 2951

PubMed: 16763562
DOI: 10.1038/sj.emboj.7601171

Chain : A, B, C, D
UniProt : P25522 (MNME_ECOLI)
Reaction : -