Brand  (β version)

PDB ID: 2GAG

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Ligands
Code Name Style Show Link
FAD Flavin-adenine dinucleotide PoSSuM
FMN Flavin mononucleotide PoSSuM
FOA 2-furoic acid PoSSuM
GOL Glycerol PoSSuM
NAD Nicotinamide-adenine-dinucleotide PoSSuM
SO3 Sulfite ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 2GAG   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 Å resolution
Release Data : 2006-08-08
Compound :
mol_id molecule chains
1 heterotetrameric sarcosine oxidase alpha-subunit A
mol_id molecule chains
2 heterotetrameric sarcosine oxidase beta-subunit B
mol_id molecule chains
3 heterotetrameric sarcosine oxidase gamma-subunit C
mol_id molecule chains
4 heterotetrameric sarcosine oxidase delta-subunit D
Source :
mol_id organism_scientific expression_system
1 Stenotrophomonas maltophilia  (taxid:40324) Escherichia coli BL21  (taxid:511693)
gene: soxA
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pET28b
mol_id organism_scientific expression_system
2 Stenotrophomonas maltophilia  (taxid:40324) Escherichia coli BL21  (taxid:511693)
gene: soxB
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pET28b
mol_id organism_scientific expression_system
3 Stenotrophomonas maltophilia  (taxid:40324) Escherichia coli BL21  (taxid:511693)
gene: soxG
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pET28b
mol_id organism_scientific expression_system
4 Stenotrophomonas maltophilia  (taxid:40324) Escherichia coli BL21  (taxid:511693)
gene: soxD
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pET28b
Authors : Chen, Z.W., Hassan-Abdulah, A., Zhao, G., Jorns, M.S., Mathews, F.S.
Keywords : Sarcosine oxidase, Flavoenzyme, Electron transfer, Folate-methylating enzyme, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Heterotetrameric sarcosine oxidase: structure of a diflavin metalloenzyme at 1.85 a resolution.

Chen, Z.W.,Hassan-Abdulah, A.,Zhao, G.  et al.
(2006)  J.Mol.Biol.  360 : 1000 - 1018

PubMed: 16820168
DOI: 10.1016/j.jmb.2006.05.067

Cloning, expression and crystallization of heterotetrameric sarcosine oxidase from Pseudomonas maltophilia


(2005)  Protein Expr.Purif.  43 : 33 - 43

PubMed: 15922624
DOI: 10.1016/j.pep.2005.03.023

Reaction

Chain : A
UniProt : Q3ZDQ8 (Q3ZDQ8_STEMA)
Reaction : -
Chain : D
UniProt : Q3ZDQ9 (Q3ZDQ9_STEMA)
Reaction : -