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Ligands
Code Name Style Show Link
EDO 1,2-ethanediol
MG Magnesium ion
Non-standard Residues
Code Name Show
C12 2-(1-amino-2-hydroxypropyl)-4-(4-hydroxybenzyl)-1-(2-oxoethyl)-1h-imidazol-5-olate
Glycosylation
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Modification
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Code : 2FZU   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Reduced enolate chromophore intermediate for GFP variant
Release Data : 2006-03-14
Compound :
mol_id molecule chains
1 Green fluorescent protein A
mutation: F64L, S65T, F99S, M153T, V163A
Source :
mol_id organism_scientific expression_system
1 Aequorea victoria  (taxid:6100) Escherichia coli BL21(DE3)  (taxid:469008)
gene: GFP
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET11a
Authors : Barondeau, D.P., Tainer, J.A., Getzoff, E.D.
Keywords : chromophore, intermediate, biosynthesis, post-translational modification, high resolution, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.25 Å )
Citation :

Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.

Barondeau, D.P.,Tainer, J.A.,Getzoff, E.D.
(2006)  J.Am.Chem.Soc.  128 : 3166 - 3168

PubMed: 16522096
DOI: 10.1021/ja0552693

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -