Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
DC 2'-deoxycytidine-5'-monophosphate
SO4 Sulfate ion
Code : 2FEO
Header : SIGNALING PROTEIN,TRANSFERASE
Title : Mutant R188M of The Cytidine Monophosphate Kinase from E. coli complexed with dCMP
Release Data : 2006-01-10
Compound :
mol_id molecule chains synonym
1 Cytidylate kinase A Cytidine monophosphate kinase; CK; CMP kinase; Protein mssA; p25
ec: 2.7.4.14
mutation: R188M
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: cmk, mssA
expression_system_strain: CJ236
expression_system_vector_type: plasmid
expression_system_plasmid: pHS210
other_details: used helper phage M13K07 to perform the single mutation
Authors : Ofiteru, A., Bucurenci, N., Alexov, E., Bertrand, T., Briozzo, P., Munier-Lehmann, H., Tourneux, L., Barzu, O., Gilles, A.M.
Keywords : NUCLEOTIDE MONOPHOSPHATE KINASE; TRANSFERASE, SIGNALING PROTEIN, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.8 Å )
Citation :

Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase.

Ofiteru, A.,Bucurenci, N.,Alexov, E.  et al.
(2007)  Febs J.  274 : 3363 - 3373

PubMed: 17542990
DOI: 10.1111/j.1742-4658.2007.05870.x

Chain : A
UniProt : P0A6I0 (KCY_ECOLI)
Reaction : ATP + (d)CMP = ADP + (d)CDP.