Brand  (β version)

PDB ID: 2F8P

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
CEI N-[3-benzyl-5-(4-hydroxyphenyl)pyrazin-2-yl]-2-(4-hydroxyphenyl)acetamide PoSSuM
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Structure summary

Code : 2F8P   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Crystal structure of obelin following Ca2+ triggered bioluminescence suggests neutral coelenteramide as the primary excited state
Release Data : 2006-02-14
Compound :
mol_id molecule chains synonym
1 Obelin A OBL
mutation: S2A
Source :
mol_id organism_scientific expression_system
1 Obelia longissima  (taxid:32570) Escherichia coli  (taxid:562)
gene: OBELIN
expression_system_strain: BL21(DE3)-Gold
expression_system_vector_type: PLASMID
expression_system_plasmid: pET19
Authors : Liu, Z.J., Stepanyuk, G.A., Vysotski, E.S., Lee, J., Wang, B.C., Southeast Collaboratory for Structural Genomics (SECSG)
Keywords : helix-turn-helix, Structural Genomics, PSI, Protein Structure Initiative, Southeast Collaboratory for Structural Genomics, SECSG, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.93 Å )
Citation :

Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state.

Liu, Z.J.,Stepanyuk, G.A.,Vysotski, E.S.  et al.
(2006)  Proc.Natl.Acad.Sci.Usa  103 : 2570 - 2575

PubMed: 16467137
DOI: 10.1073/pnas.0511142103

Structure of the Ca2+-Regulated Photoprotein Obelin at 1.7 Resolution Determined Directly From its Sulfur Substructure

Liu, Z.J.,Vysotski, E.S.,Chen, C.J.  et al.
(2000)  Protein Sci.  9 : 2085 - 2093

PubMed: 11152120

Structural Basis for the Emission of Violet Bioluminescence from a W92F Obelin Mutant

Deng, L.,Vysotski, E.S.,Liu, Z.J.  et al.
(2001)  FEBS Lett.  506 : 281 - 285

PubMed: 11602262
DOI: 10.1016/S0014-5793(01)02937-4

Violet Bioluminescence and Fast Kinetics from W92F Obelin: Structure-Based Proposals for the Bioluminescence Triggering and the Identification of the Emitting Species

Vysotski, E.S.,Liu, Z.J.,Markova, S.V.  et al.
(2003)  Biochemistry  42 : 6013 - 6024

PubMed: 12755603
DOI: 10.1021/bi027258h

Atomic Resolution Structure of Obelin: Soaking with Calcium Enhances Electron Density of the Second Oxygen Atom Substituted at the C2-Position of Coelenterazine

Liu, Z.J.,Vysotski, E.S.,Deng, L.  et al.
(2003)  Biochem.Biophys.Res.Commun.  311 : 433 - 439

PubMed: 14592432
DOI: 10.1016/j.bbrc.2003.09.231

Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence

Deng, L.,Markova, S.V.,Vysotski, E.S.  et al.
(2004)  J.Biol.Chem.  279 : 33647 - 33652

PubMed: 15155735
DOI: 10.1074/jbc.M402427200

Reaction

Chain : A
UniProt : Q27709 (OBL_OBELO)
Reaction : -