Brand  (β version)

PDB ID: 2F8G

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Ligands
Code Name Style Show Link
017 (3r,3as,6ar)-hexahydrofuro[2,3-B]furan-3-yl(1s,2r)-3-[[(4-aminophenyl)sulfonyl](isobutyl)amino]-1-benzyl-2-hydroxypropylcarbamate PoSSuM
ACY Acetic acid PoSSuM
CL Chloride ion PoSSuM
GOL Glycerol PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 2F8G   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : HIV-1 protease mutant I50V complexed with inhibitor TMC114
Release Data : 2006-03-07
Compound :
mol_id molecule chains
1 POL POLYPROTEIN A,B
ec: 3.4.23.16
fragment: PROTEASE (RETROPEPSIN)
mutation: Q7K, L33I, I50V, L63I, C67A, C95A
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus 1  (taxid:11676) Escherichia coli BL21(DE3)  (taxid:469008)
strain: BH5 isolate
gene: POL
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET11A
Authors : Kovalevsky, A.Y., Weber, I.T.
Keywords : HIV-1 protease-inhibitor complex, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.22 Å )
Citation :

Effectiveness of Nonpeptide Clinical Inhibitor TMC-114 on HIV-1 Protease with Highly Drug Resistant Mutations D30N, I50V, and L90M.

Kovalevsky, A.Y.,Tie, Y.,Liu, F.  et al.
(2006)  J.Med.Chem.  49 : 1379 - 1387

PubMed: 16480273
DOI: 10.1021/jm050943c

Reaction

Chain : A, B
UniProt : P04587 (POL_HV1B5)
Reaction: EC: Evidence:
Physiological Direction:
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro. 3.4.23.16 PROSITE-ProRule:PRU00275
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Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 3.1.26.13 ECO:0000250
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3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 3.1.13.2 ECO:0000250
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a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.49 PROSITE- ProRule:PRU00405
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a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 PROSITE- ProRule:PRU00405
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