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Ligands
Code Name Style Show Link
CAG Guanosine 5'-triphosphate p3-[1-(2-nitrophenyl)ethyl ester]
MG Magnesium ion
XY2 N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine
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Code : 2EVW   PDBj   RCSB PDB   PDBe
Header : SIGNALING PROTEIN
Title : Crystal structure analysis of a fluorescent form of H-Ras p21 in complex with R-caged GTP
Release Data : 2006-05-30
Compound :
mol_id molecule chains synonym
1 GTPase HRas X Transforming protein p21, H-Ras-1, c-H-ras
fragment: truncated form residues 1-166
mutation: Y32C,C118S
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: HRAS, HRAS1
expression_system_strain: CK600K
expression_system_vector_type: Plasmid
expression_system_plasmid: ptac ras
Authors : Klink, B.U.
Keywords : guanine nucleotide binding protein, fluorescence, caged GTP, SIGNALING PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.050 Å )
Citation :

A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction.

Klink, B.U.,Goody, R.S.,Scheidig, A.J.
(2006)  Biophys.J.  91 : 981 - 992

PubMed: 16698776
DOI: 10.1529/biophysj.105.078931

Chain : X
UniProt : P01112 (RASH_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
GTP + H2O = GDP + H(+) + phosphate 3.6.5.2 PubMed:9020151
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