Brand  (β version)

PDB ID: 2DD4

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Ligands
Code Name Style Show Link
TLA L(+)-tartaric acid PoSSuM
FRU Beta-D-fructofuranose PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2DD4   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus recombinant apo-enzyme
Release Data : 2007-01-30
Compound :
mol_id molecule chains synonym
1 Thiocyanate hydrolase alpha subunit A,D,G,J SCNase alpha subunit
ec: 3.5.5.8
mol_id molecule chains synonym
2 Thiocyanate hydrolase beta subunit B,E,H,K SCNase beta subunit
ec: 3.5.5.8
mol_id molecule chains synonym
3 Thiocyanate hydrolase gamma subunit C,F,I,L SCNase gamma subunit
ec: 3.5.5.8
Source :
mol_id organism_scientific expression_system
1 Thiobacillus thioparus  (taxid:931) Escherichia coli BL21(DE3)  (taxid:469008)
strain: THI115
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30A
mol_id organism_scientific expression_system
2 Thiobacillus thioparus  (taxid:931) Escherichia coli BL21(DE3)  (taxid:469008)
strain: THI115
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30A
mol_id organism_scientific expression_system
3 Thiobacillus thioparus  (taxid:931) Escherichia coli BL21(DE3)  (taxid:469008)
strain: THI115
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30A
Authors : Arakawa, T., Kawano, Y., Kataoka, S., Katayama, Y., Kamiya, N., Yohda, M., Odaka, M.
Keywords : hydrolase, cobalt, metalloprotein, sulfenic acid, sulfinic acid, nitrile hydratase, thiocyanate, carbonyl sulfide, claw setting, protein, enzyme, complex, model complex, non-corrin
Exp. method : X-RAY DIFFRACTION ( 2.06 Å )
Citation :

Structure of thiocyanate hydrolase: a new nitrile hydratase family protein with a novel five-coordinate cobalt(III) center.

Arakawa, T.,Kawano, Y.,Kataoka, S.  et al.
(2007)  J.Mol.Biol.  366 : 1497 - 1509

PubMed: 17222425
DOI: 10.1016/j.jmb.2006.12.011

Reaction

Chain : A, D, G, J
UniProt : O66187 (SCNA_THITI)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + H2O + thiocyanate = carbonyl sulfide + NH4(+) 3.5.5.8 -
-
Chain : B, E, H, K
UniProt : O66186 (SCNB_THITI)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + H2O + thiocyanate = carbonyl sulfide + NH4(+) 3.5.5.8 -
-
Chain : C, F, I, L
UniProt : O66188 (SCNC_THITI)
Reaction: EC: Evidence:
Physiological Direction:
2 H(+) + H2O + thiocyanate = carbonyl sulfide + NH4(+) 3.5.5.8 -
-