Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
FRU Fructose
TLA L(+)-tartaric acid
Code : 2DD4
Header : HYDROLASE
Title : Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus recombinant apo-enzyme
Release Data : 2007-01-30
Compound :
mol_id molecule chains synonym
1 Thiocyanate hydrolase alpha subunit A,D,G,J SCNase alpha subunit
ec: 3.5.5.8
mol_id molecule chains synonym
2 Thiocyanate hydrolase beta subunit B,E,H,K SCNase beta subunit
ec: 3.5.5.8
mol_id molecule chains synonym
3 Thiocyanate hydrolase gamma subunit C,F,I,L SCNase gamma subunit
ec: 3.5.5.8
Source :
mol_id organism_scientific expression_system
1 Thiobacillus thioparus  (taxid:931) Escherichia coli BL21(DE3)  (taxid:469008)
strain: THI115
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30A
mol_id organism_scientific expression_system
2 Thiobacillus thioparus  (taxid:931) Escherichia coli BL21(DE3)  (taxid:469008)
strain: THI115
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30A
mol_id organism_scientific expression_system
3 Thiobacillus thioparus  (taxid:931) Escherichia coli BL21(DE3)  (taxid:469008)
strain: THI115
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30A
Authors : Arakawa, T., Kawano, Y., Kataoka, S., Katayama, Y., Kamiya, N., Yohda, M., Odaka, M.
Keywords : hydrolase, cobalt, metalloprotein, sulfenic acid, sulfinic acid, nitrile hydratase, thiocyanate, carbonyl sulfide, claw setting, protein, enzyme, complex, model complex, non-corrin
Exp. method : X-RAY DIFFRACTION ( 2.06 Å )
Citation :

Structure of thiocyanate hydrolase: a new nitrile hydratase family protein with a novel five-coordinate cobalt(III) center.

Arakawa, T.,Kawano, Y.,Kataoka, S.  et al.
(2007)  J.Mol.Biol.  366 : 1497 - 1509

PubMed: 17222425
DOI: 10.1016/j.jmb.2006.12.011

Chain : A, D, G, J
UniProt : O66187 (SCNA_THITI)
Reaction : Thiocyanate + 2 H(2)O = carbonyl sulfide + NH(3) + HO(-).
Chain : B, E, H, K
UniProt : O66186 (SCNB_THITI)
Reaction : Thiocyanate + 2 H(2)O = carbonyl sulfide + NH(3) + HO(-).
Chain : C, F, I, L
UniProt : O66188 (SCNC_THITI)
Reaction : Thiocyanate + 2 H(2)O = carbonyl sulfide + NH(3) + HO(-).