Brand  (β version)

PDB ID: 2DBW

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
GGL Gamma-L-glutamic acid PoSSuM
GOL Glycerol PoSSuM
Modified Residues
Code Name Link
MSE Selenomethionine PoSSuM

Structure summary

Code : 2DBW   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure of Gamma-glutamyltranspeptidase from Escherichia coli Acyl-Enzyme Intermediate
Release Data : 2006-04-18
Compound :
mol_id molecule chains
1 Gamma-glutamyltranspeptidase A,C
ec: 2.3.2.2
fragment: LARGE SUBUNIT
mol_id molecule chains
2 Gamma-glutamyltranspeptidase B,D
ec: 2.3.2.2
fragment: SMALL SUBUNIT
Source :
mol_id organism_scientific expression_system
1 Escherichia coli K12  (taxid:83333) Escherichia coli  (taxid:562)
strain: K-12
gene: GGT
expression_system_strain: SH1603
expression_system_vector_type: PLASMID
expression_system_plasmid: PSH1291
mol_id organism_scientific expression_system
2 Escherichia coli K12  (taxid:83333) Escherichia coli  (taxid:562)
strain: K-12
gene: GGT
expression_system_strain: SH1603
expression_system_vector_type: PLASMID
expression_system_plasmid: PSH1291
Authors : Okada, T., Wada, K., Fukuyama, K.
Keywords : gamma-glutamyltransferase, ggt, gamma-gtp, glutathione, acyl-enzyme intermediate, Transferase
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Crystal structures of gamma-glutamyltranspeptidase from Escherichia coli, a key enzyme in glutathione metabolism, and its reaction intermediate

Okada, T.,Suzuki, H.,Wada, K.  et al.
(2006)  Proc.Natl.Acad.Sci.USA  103 : 6471 - 6476

PubMed: 16618936
DOI: 10.1073/pnas.0511020103

Reaction

Chain : B, D
UniProt : P18956 (GGT_ECOLI)
Reaction : A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.
Glutathione + H(2)O = L-cysteinylglycine + L- glutamate.