Brand  (β version)

PDB ID: 2CLM

Hetero Atom Contents

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hetatm:

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information
centroid:
interaction residue:

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Ligands
Code Name Link
F6F 2-{[4-(trifluoromethoxy)benzoyl]amino}ethyl dihydrogen phosphate PoSSuM
NA Sodium ion PoSSuM
PLS [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine PoSSuM

Structure summary

Code : 2CLM
Header : LYASE
Title : Tryptophan Synthase (external aldimine state) in complex with N-(4'- trifluoromethoxybenzoyl)-2-amino-1-ethylphosphate (F6F)
Release Data : 2007-06-12
Compound :
mol_id molecule chains
1 TRYPTOPHAN SYNTHASE ALPHA CHAIN A
ec: 4.2.1.20
mol_id molecule chains
2 TRYPTOPHAN SYNTHASE BETA CHAIN B
ec: 4.2.1.20
Source :
mol_id organism_scientific expression_system
1 SALMONELLA TYPHIMURIUM  (taxid:602) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: CB149
expression_system_plasmid: PSTB7
mol_id organism_scientific expression_system
2 SALMONELLA TYPHIMURIUM  (taxid:602) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: CB149
expression_system_plasmid: PSTB7
Authors : Ngo, H., Kimmich, N., Harris, R., Niks, D., Blumenstein, L., Kulik, V., Barends, T.R., Schlichting, I., Dunn, M.F.
Keywords : AROMATIC AMINO ACID BIOSYNTHESIS, CARBON-OXYGEN LYASE, AMINO-ACID BIOSYNTHESIS, TRYPTOPHAN BIOSYNTHESIS, LYASE, ALLOSTERIC ENZYME, PYRIDOXAL PHOSPHATE
Exp. method : X-RAY DIFFRACTION ( 1.51 Å )
Citation :

Allosteric Regulation of Substrate Channeling in Tryptophan Synthase: Modulation of the L-Serine Reaction in Stage I of the Beta-Reaction by Alpha-Site Ligands.

Ngo, H.,Kimmich, N.,Harris, R.  et al.
(2007)  Biochemistry  46 : 7740

PubMed: 17559232
DOI: 10.1021/BI7003872

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction : L-serine + 1-C-(indol-3-yl)glycerol 3- phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H(2)O.
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction : L-serine + 1-C-(indol-3-yl)glycerol 3- phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H(2)O.