Brand  (β version)

PDB ID: 2CLH

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Ligands
Code Name Style Show Link
F19 2-[(2-naphthylsulfonyl)amino]ethyl dihydrogen phosphate PoSSuM
NA Sodium ion PoSSuM
PLP Pyridoxal-5'-phosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2CLH   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Tryptophan Synthase in complex with (naphthalene-2'-sulfonyl)-2-amino- 1-ethylphosphate (F19)
Release Data : 2007-06-12
Compound :
mol_id molecule chains
1 Tryptophan synthase alpha chain A
ec: 4.2.1.20
mol_id molecule chains
2 Tryptophan synthase beta chain B
ec: 4.2.1.20
Source :
mol_id organism_scientific expression_system
1 Salmonella typhimurium  (taxid:602) ESCHERICHIA COLI  (taxid:562)
gene: trpA, STM1727
expression_system_strain: CB149
expression_system_plasmid: PSTB7
mol_id organism_scientific expression_system
2 Salmonella typhimurium  (taxid:602) Escherichia coli  (taxid:562)
gene: trpB, STM1726
expression_system_strain: CB149
Authors : Ngo, H., Harris, R., Kimmich, N., Casino, P., Niks, D., Blumenstein, L., Barends, T.R., Kulik, V., Weyand, M., Schlichting, I., Dunn, M.F.
Keywords : AROMATIC AMINO ACID BIOSYNTHESIS, TRYPTOPHAN BIOSYNTHESIS, CRBON- OXYGEN LYASE, AMINO-ACID BIOSYNTHESIS, LYASE, ALLOSTERIC ENZYME, PYRIDOXAL PHOSPHATE
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex.

Ngo, H.,Harris, R.,Kimmich, N.  et al.
(2007)  Biochemistry  46 : 7713 - 7727

PubMed: 17559195
DOI: 10.1021/bi700385f

Reaction

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan 4.2.1.20 HAMAP-Rule:MF_00131
-
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction: EC: Evidence:
Physiological Direction:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D- glyceraldehyde 3-phosphate + H2O + L-tryptophan 4.2.1.20 -
-