Brand  (β version)

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interaction residue:

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Ligands
Code Name Link
NA Sodium ion
F6F 2-{[4-(trifluoromethoxy)benzoyl]amino}ethyl dihydrogen phosphate
PLP Pyridoxal-5'-phosphate
Code : 2CLF
Header : LYASE
Title : Tryptophan Synthase in complex with N-(4'-trifluoromethoxybenzoyl)-2- amino-1-ethylphosphate (F6) - highF6 complex
Release Data : 2007-06-26
Compound :
mol_id molecule chains
1 TRYPTOPHAN SYNTHASE ALPHA CHAIN A
ec: 4.2.1.20
mol_id molecule chains
2 TRYPTOPHAN SYNTHASE BETA CHAIN B
ec: 4.2.1.20
other_details: INTERNAL ALDIMINE FORMED BETWEEN B-K87 AND C4 OF PYRIDOXAL-5'-PHOSPHATE
Source :
mol_id organism_scientific expression_system
1 SALMONELLA TYPHIMURIUM  (taxid:602) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: CB149
expression_system_plasmid: PSTB7
mol_id organism_scientific expression_system
2 SALMONELLA TYPHIMURIUM  (taxid:602) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: CB149
expression_system_plasmid: PSTB7
Authors : Ngo, H., Harris, R., Kimmich, N., Casino, P., Niks, D., Blumenstein, L., Barends, T.R., Kulik, V., Weyand, M., Schlichting, I., Dunn, M.F.
Keywords : AROMATIC AMINO ACID BIOSYNTHESIS, TRYPTOPHAN BIOSYNTHESIS, CRBON- OXYGEN LYASE, AMINO-ACID BIOSYNTHESIS, LYASE, ALLOSTERIC ENZYME, PYRIDOXAL PHOSPHATE
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Synthesis and Characterization of Allosteric Probes of Substrate Channeling in the Tryptophan Synthase Bienzyme Complex.

Ngo, H.,Harris, R.,Kimmich, N.  et al.
(2007)  Biochemistry  46 : 7713

PubMed: 17559195
DOI: 10.1021/BI700385F

Chain : A
UniProt : P00929 (TRPA_SALTY)
Reaction : L-serine + 1-C-(indol-3-yl)glycerol 3- phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H(2)O.
Chain : B
UniProt : P0A2K1 (TRPB_SALTY)
Reaction : L-serine + 1-C-(indol-3-yl)glycerol 3- phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H(2)O.