Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
CA Calcium ion
DG 2'-deoxyguanosine-5'-monophosphate
Modified Residues
Code Name Link
8OG 8-oxo-2'-deoxy-guanosine-5'-monophosphate
Code : 2C28
Header : POLYMERASE
Title : Efficient and High Fidelity Incorporation of dCTP Opposite 7,8- Dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA Polymerase Dpo4
Release Data : 2005-11-29
Compound :
mol_id molecule chains synonym
1 DNA POLYMERASE IV A POL IV
ec: 2.7.7.7
mol_id molecule chains
2 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP *TP*TP*C)-3' P
other_details: 13-MER PRIMER
mol_id molecule chains
3 5'-D(TP*CP*AP*C 8OGP*GP*AP*AP*TP*CP*CP*TP *TP*CP*CP*CP*CP*C)-3' T
other_details: 18-MER TEMPLATE CONTAINING A 7,8-DIHYDRO-8-OXODEOXYGUANOSINE
Source :
mol_id organism_scientific expression_system
1 SULFOLOBUS SOLFATARICUS  (taxid:273057) ESCHERICHIA COLI  (taxid:469008)
strain: P2
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET22B/DPO4-NHIS
other_details: GENE DPO4
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Irimia, A., Loukachevitch, L.V., Egli, M.
Keywords : POLYMERASE, 7, 8-DIHYDRO-8-OXODEOXYGUANOSINE, P2 DNA POLYMERASE IV, TRANSLESION DNA POLYMERASE, DDGTP, NUCLEOTIDYLTRANSFERASE, TRANSFERASE, DNA DAMAGE, DNA REPAIR, DNA REPLICATION, DNA- BINDING, DNA-DIRECTED DNA POLYMERASE, MAGNESIUM, METAL- BINDING, MUTATOR PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.27 Å )
Citation :

Efficient and High Fidelity Incorporation of Dctp Opposite 7,8-Dihydro-8-Oxodeoxyguanosine by Sulfolobus Solfataricus DNA Polymerase Dpo4

Zang, H.,Irimia, A.,Choi, J.-Y.  et al.
(2006)  J.Biol.Chem.  281 : 2358

PubMed: 16306039
DOI: 10.1074/JBC.M510889200

Chain : A
UniProt : Q97W02 (DPO4_SULSO)
Reaction : Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).