Brand  (β version)

PDB ID: 2C22

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
DGT 2'-deoxyguanosine-5'-triphosphate PoSSuM
Non-standard Residues
Code Name Show
8OG 8-oxo-2'-deoxy-guanosine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 2C22   PDBj   RCSB PDB   PDBe
Header : POLYMERASE
Title : Efficient and High Fidelity Incorporation of dCTP Opposite 7,8- Dihydro-8-oxodeoxyguanosine by Sulfolobus solfataricus DNA Polymerase Dpo4
Release Data : 2005-11-29
Compound :
mol_id molecule chains synonym
1 DNA POLYMERASE IV A POL IV
ec: 2.7.7.7
mol_id molecule chains
2 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP *TP*TP*C)-3' P
mol_id molecule chains
3 5'-D(TP*CP*AP*C 8OGP*GP*AP*AP*TP*CP*CP*TP *TP*CP*CP*CP*CP*C)-3' T
Source :
mol_id organism_scientific expression_system
1 SULFOLOBUS SOLFATARICUS  (taxid:273057) ESCHERICHIA COLI  (taxid:469008)
strain: P2
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET22B/DPO4-NHIS
other_details: GENE DPO4
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Irimia, A., Loukachevitch, L.V., Egli, M.
Keywords : POLYMERASE, P2 DNA POLYMERASE IV, 7, 8-DIHYDRO-8-OXODEOXYGUANOSINE, TRANSLESION DNA POLYMERASE, NUCLEOTIDYLTRANSFERASE, TRANSFERASE, DNA DAMAGE, DNA REPAIR, DNA REPLICATION, DNA- BINDING, DNA-DIRECTED DNA POLYMERASE, MAGNESIUM, METAL - BINDING, MUTATOR PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.56 Å )
Citation :

Efficient and High Fidelity Incorporation of Dctp Opposite 7,8-Dihydro-8-Oxodeoxyguanosine by Sulfolobus Solfataricus DNA Polymerase Dpo4

Zang, H.,Irimia, A.,Choi, J.-Y.  et al.
(2006)  J.Biol.Chem.  281 : 2358

PubMed: 16306039
DOI: 10.1074/JBC.M510889200

Reaction

Chain : A
UniProt : Q97W02 (DPO4_SACS2)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-