Brand  (β version)

PDB ID: 2BR0

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
DG 2'-deoxyguanosine-5'-monophosphate PoSSuM
Non-standard Residues
Code Name Show
GNE 1,n2-ethenoguanine
Glycosylation
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Structure summary

Code : 2BR0   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : DNA Adduct Bypass Polymerization by Sulfolobus solfataricus Dpo4. Analysis and Crystal Structures of Multiple Base-Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine
Release Data : 2005-06-23
Compound :
mol_id molecule chains synonym
1 DNA POLYMERASE IV A POL IV
ec: 2.7.7.7
mol_id molecule chains
2 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP *TP*TP*C)-3' P
mol_id molecule chains
3 5'-D(*TP*CP*AP*CP*GNEP*GP*AP*AP*TP*CP*CP *TP*TP*CP*CP*CP*CP*C)-3' T
other_details: 18-MER DNA TEMPLATE CONTAINING 1, N2-ETHENOGUANINE ADDUCT
Source :
mol_id organism_scientific organism_common expression_system
1 SULFOLOBUS SOLFATARICUS  (taxid:273057) SULFOLOBUS SOLFATARICUS ESCHERICHIA COLI  (taxid:469008)
strain: P2
gene: DPO4
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET22B/DPO4-NHIS
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Irimia, A., Loukachevitch, L.V., Egli, M.
Keywords : P2 DNA POLYMERASE IV, 1N2-ETHENOGUANINE ADDUCT, TRANSLESION DNA POLYMERASE, DATP, NUCLEOTIDYLTRANSFERASE, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.17 Å )
Citation :

DNA Adduct Bypass Polymerization by Sulfolobus Solfataricus DNA Polymerase Dpo4: Analysis and Crystal Structures of Multiple Base Pair Substitution and Frameshift Products with the Adduct 1,N2-Ethenoguanine.

Zang, H.,Goodenough, A.K.,Choi, J.Y.  et al.
(2005)  J.Biol.Chem.  280 : 29750

PubMed: 15965231
DOI: 10.1074/JBC.M504756200

Reaction

Chain : A
UniProt : Q97W02 (DPO4_SACS2)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 -
-