Brand  (β version)

PDB ID: 2B0Q

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
ADP Adenosine-5'-diphosphate PoSSuM
MG Magnesium ion PoSSuM
NMY Neomycin PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 2B0Q   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure Of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Neomycin B Complex
Release Data : 2005-09-27
Compound :
mol_id molecule chains synonym
1 Aminoglycoside 3'-phosphotransferase A Kanamycin kinase, type III, Neomycin-kanamycin phosphotransferase, type III, APH3', III
ec: 2.7.1.95
Source :
mol_id organism_scientific expression_system
1 Enterococcus faecalis  (taxid:1351) Escherichia coli BL21  (taxid:511693)
gene: aphA
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pETSACG1
Authors : Fong, D.H., Berghuis, A.M.
Keywords : protein kinase-like, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.700 Å )
Citation :

Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry.

Fong, D.H.,Berghuis, A.M.
(2002)  Embo J.  21 : 2323 - 2331

PubMed: 12006485
DOI: 10.1093/emboj/21.10.2323

Reaction

Chain : A
UniProt : P0A3Y5 (KKA3_ENTFL)
Reaction: EC: Evidence:
Physiological Direction:
ATP + kanamycin A = ADP + H(+) + kanamycin 3'-phosphate 2.7.1.95 -
-