Brand  (β version)

PDB ID: 2ASJ

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
Non-standard Residues
Code Name Show
DDG 2',3'-dideoxy-guanosine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 2ASJ   PDBj   RCSB PDB   PDBe
Header : transferase/DNA
Title : oxoG-modified Preinsertion Binary Complex
Release Data : 2006-01-10
Compound :
mol_id molecule chains
1 5'-D(*GP*GP*TP*TP*GP*GP*AP*TP*GP*GP*TP*AP*(DDG))-3' D,H
other_details: Primer Strand (dideoxy-terminated at 3'-end)
mol_id molecule chains
2 5'-D(*CP*TP*AP*AP*CP*(8OG)*CP*TP*AP*CP*CP*AP*TP*CP*CP*AP*AP*CP*C)-3' E,J
other_details: oxoG-modified Template Strand
mol_id molecule chains synonym
3 DNA polymerase IV A,B Pol IV
ec: 2.7.7.7
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Sulfolobus solfataricus  (taxid:2287) Escherichia coli  (taxid:562)
gene: dbh, dpo4
expression_system_strain: BL21(DE3)-RIL (Stratagene)
expression_system_vector_type: plasmid
expression_system_plasmid: pET-28a
Authors : Rechkoblit, O., Malinina, L., Cheng, Y., Kuryavyi, V., Broyde, S., Geacintov, N.E., Patel, D.J.
Keywords : DNA polymerase, 8-oxoguanine, Y-family, lesion bypass, transferase-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.35 Å )
Citation :

Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion

Rechkoblit, O.,Malinina, L.,Cheng, Y.  et al.
(2006)  Plos Biol.  4 : 1 - 18

PubMed: 16379496
DOI: 10.1371/journal.pbio.0040011

Reaction

Chain : A, B
UniProt : Q97W02 (DPO4_SACS2)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 -
-
Cofactor: Evidence: Note:
Mg(2+) - Binds 2 magnesium ions per subunit.