Brand  (β version)

PDB ID: 2AN3

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Ligands
Code Name Style Show Link
SAH S-adenosyl-L-homocysteine PoSSuM
CTL Cis-(1r,2s)-2-amino-1,2,3,4-tetrahydronaphthalen-1-ol PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2AN3   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Structure of PNMT with S-adenosyl-L-homocysteine and the semi-rigid analogue acceptor substrate cis-(1R,2S)-2-amino-1-tetralol.
Release Data : 2006-03-14
Compound :
mol_id molecule chains synonym
1 Phenylethanolamine N-methyltransferase A,B PNMTase, Noradrenaline N-methyltransferase
ec: 2.1.1.28
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: PNMT
expression_system_strain: BL21(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pET17PNMT-his
Authors : Gee, C.L., Tyndall, J.D.A., Grunewald, G.L., Wu, Q., McLeish, M.J., Martin, J.L.
Keywords : methyltransferase, substrate structure, S-adenosyl-L-methionine, adrenaline synthesis, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.200 Å )
Citation :

Mode of binding of methyl acceptor substrates to the adrenaline-synthesizing enzyme phenylethanolamine N-methyltransferase: implications for catalysis

Gee, C.L.,Tyndall, J.D.A.,Grunewald, G.L.  et al.
(2005)  Biochemistry  44 : 16875 - 16885

PubMed: 16363801
DOI: 10.1021/bi051636b

Reaction

Chain : A, B
UniProt : P11086 (PNMT_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
phenylethanolamine + S-adenosyl-L-methionine = H(+) + N- methylphenylethanolamine + S-adenosyl-L-homocysteine 2.1.1.28 PubMed:16363801, PubMed:8812853
left-to-right PubMed:16363801
(R)-noradrenaline + S-adenosyl-L-methionine = (R)-adrenaline + H(+) + S-adenosyl-L-homocysteine 2.1.1.28 PubMed:20496117
left-to-right
(R)-normetanephrine + S-adenosyl-L-methionine = (R)- metanephrine + H(+) + S-adenosyl-L-homocysteine - UniProtKB:P10938
left-to-right
(R)-octopamine + S-adenosyl-L-methionine = (R)-synephrine + H(+) + S-adenosyl-L-homocysteine - PubMed:16277617, PubMed:16363801
left-to-right PubMed:16363801