Brand  (β version)

PDB ID: 2AGP

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
MG Magnesium ion PoSSuM
DGT 2'-deoxyguanosine-5'-triphosphate PoSSuM
Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 2AGP   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis
Release Data : 2005-09-06
Compound :
mol_id molecule chains
1 DNA (5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*TP*TP*(DOC)-3') C,E
mol_id molecule chains
2 DNA (5'-D(*TP*TP*TP*TP*GP*AP*AP*TP*CP*CP*TP*TP*CP*CP*CP*CP*C)-3') D,F
mol_id molecule chains synonym
3 DNA polymerase IV A,B Pol IV
ec: 2.7.7.7
Source :
mol_id organism_scientific
1
synthetic: yes
other_details: chemically synthesized
mol_id organism_scientific
2
synthetic: yes
other_details: chemically synthesized
mol_id organism_scientific expression_system
3 Sulfolobus solfataricus  (taxid:2287) Escherichia coli  (taxid:562)
gene: dbh, dpo4
Authors : Ling, H., Yang, W.
Keywords : base stacking, fidelity, metal ion, mismatch, pyrophosphorolysis, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.900 Å )
Citation :

Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis.

Vaisman, A.,Ling, H.,Woodgate, R.  et al.
(2005)  Embo J.  24 : 2957 - 2967

PubMed: 16107880
DOI: 10.1038/sj.emboj.7600786

Reaction

Chain : A, B
UniProt : Q97W02 (DPO4_SACS2)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 -
-
Cofactor: Evidence: Note:
Mg(2+) - Binds 2 magnesium ions per subunit.