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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
CYR N~5~-[{[(2r)-2-amino-2-carboxyethyl]sulfanyl}(iminio)methyl]-L-ornithinate
Glycosylation
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Modification
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Code : 2ABR   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Structure of D280A arginine deiminase with L-arginine forming a S-alkylthiouronium reaction intermediate
Release Data : 2005-08-09
Compound :
mol_id molecule chains synonym
1 Arginine deiminase A,B,C,D ADI, Arginine dihydrolase, AD
ec: 3.5.3.6
mutation: D280A
Source :
mol_id organism_scientific expression_system
1 Pseudomonas aeruginosa  (taxid:287) Escherichia coli  (taxid:562)
gene: arcA
expression_system_strain: BL21(DE3)Star
expression_system_vector_type: plasmid
expression_system_plasmid: pET100/ADIn
Authors : Galkin, A., Lu, X., Dunaway-Mariano, D., Herzberg, O.
Keywords : arginine degradation pathway, L-arginine deiminase, catalytic mechanism, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.9 Å )
Citation :

Crystal Structures Representing the Michaelis Complex and the Thiouronium Reaction Intermediate of Pseudomonas aeruginosa Arginine Deiminase.

Galkin, A.,Lu, X.,Dunaway-Mariano, D.  et al.
(2005)  J.Biol.Chem.  280 : 34080 - 34087

PubMed: 16091358
DOI: 10.1074/jbc.M505471200

Chain : A, B, C, D
UniProt : P13981 (ARCA_PSEAE)
Reaction: EC: Evidence:
Physiological Direction:
H2O + L-arginine = L-citrulline + NH4(+) 3.5.3.6 -
-