Brand  (β version)

PDB ID: 2A8P

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
Non-standard Residues
Code Name Show
YCM S-(2-amino-2-oxoethyl)-L-cysteine
Glycosylation
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Modification
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Structure summary

Code : 2A8P   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : 2.7 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese
Release Data : 2006-03-28
Compound :
mol_id molecule chains
1 U8 snoRNA-binding protein X29 A,B
ec: 3.6.1.-
Source :
mol_id organism_scientific organism_common expression_system
1 Xenopus laevis  (taxid:8355) African clawed frog Escherichia coli  (taxid:562)
expression_system_strain: BL21(DES)pLysS
expression_system_vector_type: Plasmid
expression_system_plasmid: pET19b
Authors : Scarsdale, J.N., Peculis, B.A., Wright, H.T.
Keywords : modified nudix hydrolase fold, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.70 Å )
Citation :

Crystal structures of U8 snoRNA decapping nudix hydrolase, X29, and its metal and cap complexes

Scarsdale, J.N.,Peculis, B.A.,Wright, H.T.
(2006)  Structure  14 : 331 - 343

PubMed: 16472752
DOI: 10.1016/j.str.2005.11.010

Crystals of X29, a Xenopus Laevis U8 SnoRNA Binding Protein with Nuclear Decapping Activity

Peculis, B.A.,Scarsdale, J.N.,Wright, H.T.
(2004)  Acta Crystallogr.,Sect.D  60 : 1668 - 1669

Xenopus U8 SnoRNA Binding Protein is a Conserved Nuclear Decapping Enzyme

Ghosh, T.,Peterson, B.,Tomasevic, N.  et al.
(2004)  Mol.Cell  13 : 817 - 828

Reaction

Chain : A, B
UniProt : Q569R2 (NUD16_XENLA)
Reaction: EC: Evidence:
Physiological Direction:
a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + 2 H(+) + N(7)-methyl-GDP 3.6.1.62 PubMed:15053875, PubMed:17567574, PubMed:18820299
left-to-right PubMed:15053875, PubMed:17567574, PubMed:18820299
H2O + IDP = H(+) + IMP + phosphate 3.6.1.64 UniProtKB:Q96DE0
left-to-right
dIDP + H2O = dIMP + H(+) + phosphate 3.6.1.64 UniProtKB:Q96DE0
left-to-right
a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'- end phospho-ribonucleoside in mRNA + H(+) + NAD(+) - UniProtKB:Q96DE0
left-to-right
a 5'-end FAD-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + FMN + 2 H(+) - UniProtKB:Q96DE0
left-to-right
a 5'-end CoA-ribonucleoside in mRNA + H2O = (R)-4'- phosphopantetheine + a 5'-end phospho-adenosine-phospho- ribonucleoside in mRNA + 2 H(+) - UniProtKB:Q6P3D0
left-to-right