Brand  (β version)

PDB ID: 1YWB

Hetero Atom Contents

  The number of atoms exceeds 100,000.
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Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
NO Nitric oxide PoSSuM
PO4 Phosphate ion PoSSuM

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Structure summary

Code : 1YWB   PDBj   RCSB PDB   PDBe
Header : LIGAND BINDING PROTEIN, BLOOD CLOTTING
Title : 0.9 A Structure of NP4 from Rhodnius Prolixus complexed with NO at pH 5.6
Release Data : 2005-10-04
Compound :
mol_id molecule chains synonym
1 nitrophorin 4 A NP4
Source :
mol_id organism_scientific expression_system
1 Rhodnius prolixus  (taxid:13249) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21DE3
expression_system_vector_type: PLASMID
expression_system_plasmid: PET17B
Authors : Maes, E.M., Weichsel, A., Roberts, S.A., Montfort, W.R.
Keywords : ferrous heme; nitrogen monoxide complex; lipocalin fold; beta barrel, LIGAND BINDING PROTEIN, BLOOD CLOTTING
Exp. method : X-RAY DIFFRACTION ( 0.970 Å )
Citation :

Ultrahigh Resolution Structures of Nitrophorin 4: Heme Distortion in Ferrous CO and NO Complexes

Maes, E.M.,Roberts, S.A.,Weichsel, A.  et al.
(2005)  Biochemistry  44 : 12690 - 12699

PubMed: 16171383
DOI: 10.1021/bi0506573

Reaction

Chain : A
UniProt : Q94734 (NP4_RHOPR)
Reaction: EC: Evidence:
Physiological Direction:
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H2O 1.7.6.1 PubMed:20524697
-
Cofactor: Evidence: Note:
heme b ECO:0000269 | PubMed:20524697
Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.