Brand  (β version)

PDB ID: 1XDS

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Ligands
Code Name Style Show Link
DRA 11-deoxy-beta-rhodomycin PoSSuM
SAM S-adenosylmethionine PoSSuM
Non-standard Residues
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MSE Selenomethionine
Glycosylation
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Structure summary

Code : 1XDS   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM) and 11-deoxy-beta-rhodomycin (DbrA)
Release Data : 2004-11-23
Compound :
mol_id molecule chains
1 Protein RdmB A,B
Source :
mol_id organism_scientific expression_system
1 Streptomyces purpurascens  (taxid:1924) Escherichia coli  (taxid:562)
gene: rdmb
expression_system_vector_type: plasmid
expression_system_plasmid: pRDM16 from pGEX4T-3
Authors : Jansson, A., Koskiniemi, H., Erola, A., Wang, J., Mantsala, P., Schneider, G., Niemi, J., Structural Proteomics in Europe (SPINE)
Keywords : Anthracycline; hydroxylase; S-adenosyl-L-methionine; streptomyces; polyketide antibiotics; divergent evolution, Structural Proteomics in Europe, SPINE, Structural Genomics, transferase
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Aclacinomycin 10-Hydroxylase Is a Novel Substrate-assisted Hydroxylase Requiring S-Adenosyl-L-methionine as Cofactor

Jansson, A.,Koskiniemi, H.,Erola, A.  et al.
(2005)  J.Biol.Chem.  280 : 3636 - 3644

PubMed: 15548527
DOI: 10.1074/jbc.M412095200

Reaction

Chain : A, B
UniProt : Q54527 (RDMB_STREF)
Reaction: EC: Evidence:
Physiological Direction:
15-demethylaclacinomycin T + AH2 + O2 = 10- decarboxymethylaclacinomycin T + A + CO2 + H2O - PubMed:11004563
left-to-right
10-carboxy-13-deoxycarminomycin + AH2 + O2 + H(+) = 10- hydroxy-13-deoxycarminomycin + A + CO2 + H2O - PubMed:39308748
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10-hydroxy-13-deoxycarminomycin + S-adenosyl-L-methionine = 10-hydroxy-13-deoxydaunorubicin + S-adenosyl-L-homocysteine + H(+) - PubMed:39308748
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