Brand  (β version)

PDB ID: 1SCN

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Ligands
Code Name Style Show Link
0EF N-(tert-butoxycarbonyl)-L-alanyl-N-[(1r)-1-(carboxyamino)-2-phenylethyl]-L-prolinamide PoSSuM
CA Calcium ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1SCN   PDBj   RCSB PDB   PDBe
Header : hydrolase/hydrolase INHIBITOR
Title : INACTIVATION OF SUBTILISIN CARLSBERG BY N-(TERT-BUTOXYCARBONYL-ALANYL-PROLYL-PHENYLALANYL)-O-BENZOL HYDROXYLAMINE: FORMATION OF COVALENT ENZYME-INHIBITOR LINKAGE IN THE FORM OF A CARBAMATE DERIVATIVE
Release Data : 1994-08-31
Compound :
mol_id molecule chains
1 SUBTILISIN CARLSBERG E
ec: 3.4.21.62
Source :
mol_id organism_scientific
1 Bacillus licheniformis  (taxid:1402)
Authors : Steinmetz, A.C.U., Demuth, H.-U., Ringe, D.
Keywords : HYDROLASE-HYDROLASE INHIBITOR COMPLEX, SERINE PROTEINASE
Exp. method : X-RAY DIFFRACTION ( 1.9 Å )
Citation :

Inactivation of subtilisin Carlsberg by N-((tert-butoxycarbonyl)alanylprolylphenylalanyl)-O-benzolhydroxyl- amine: formation of a covalent enzyme-inhibitor linkage in the form of a carbamate derivative.

Steinmetz, A.C.,Demuth, H.U.,Ringe, D.
(1994)  Biochemistry  33 : 10535 - 10544

PubMed: 8068694
DOI: 10.1021/bi00200a040

Reaction

Chain : E
UniProt : P00780 (SUBC_BACLI)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. 3.4.21.62 PubMed:11109488, Ref.4
-
Cofactor: Evidence: Note:
Ca(2+) ECO:0000269 | PubMed:8512925
Binds 2 calcium ions per subunit.