Brand  (β version)

PDB ID: 1RS8

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Ligands
Code Name Style Show Link
ACT Acetate ion PoSSuM
CAC Cacodylate ion PoSSuM
DP2 L-lysyl-N~5~-[(z)-(2,2-dihydroxyhydrazino)(imino)methyl]-L-ornithinamide PoSSuM
GOL Glycerol PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1RS8   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Bovine endothelial NOS heme domain with D-lysine-D-nitroarginine amide bound
Release Data : 2004-06-15
Compound :
mol_id molecule chains synonym
1 Nitric-oxide synthase, endothelial A,B EC-NOS, NOS, type III, NOSIII, Endothelial NOS, eNOS, Constitutive NOS, cNOS
ec: 1.14.13.39
fragment: heme domain
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Cattle Pichia pastoris  (taxid:4922)
gene: endothelial nitric oxide synthase
expression_system_strain: KM71H MutS
expression_system_vector_type: integration of linearized plasmid into Pichia genome
expression_system_plasmid: pPICZ
Authors : Flinspach, M., Li, H., Jamal, J., Yang, W., Huang, H., Silverman, R.B., Poulos, T.L.
Keywords : nitric oxide synthase, oxidoreductase, heme-enzyme
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Structures of the neuronal and endothelial nitric oxide synthase heme domain with D-nitroarginine-containing dipeptide inhibitors bound.

Flinspach, M.,Li, H.,Jamal, J.  et al.
(2004)  Biochemistry  43 : 5181 - 5187

PubMed: 15122883
DOI: 10.1021/bi0361867

The novel binding mode of N-alkyl-N'-hydroxyguanidine to neuronal nitric oxide synthase provides mechanistic insights into NO biosynthesis

Li, H.,Shimizu, H.,L Flinspach, M.  et al.
(2002)  Biochemistry  41 : 13868 - 13875

DOI: 10.1021/bi020417c

Reaction

Chain : A, B
UniProt : P29473 (NOS3_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
H(+) + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP(+) + 2 nitric oxide 1.14.13.39 UniProtKB:P29474
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