Brand  (β version)

PDB ID: 1RS6

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Ligands
Code Name Style Show Link
ACT Acetate ion PoSSuM
DP2 L-lysyl-N~5~-[(z)-(2,2-dihydroxyhydrazino)(imino)methyl]-L-ornithinamide PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
MTL D-mannitol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1RS6   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Rat neuronal NOS heme domain with D-lysine-D-nitroarginine amide bound
Release Data : 2004-05-18
Compound :
mol_id molecule chains synonym
1 Nitric-oxide synthase, brain A,B NOS, type I, Neuronal NOS, N-NOS, NNOS, Constitutive NOS, NC-NOS, BNOS
ec: 1.14.13.39
fragment: heme domain
Source :
mol_id organism_scientific organism_common expression_system
1 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21(DE3)  (taxid:469008)
gene: brain
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pCWori
Authors : Flinspach, M., Li, H., Jamal, J., Yang, W., Huang, H., Silverman, R.B., Poulos, T.L.
Keywords : nitric oxide synthase, oxidoreductase, heme-enzyme
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Structures of the Neuronal and Endothelial Nitric Oxide Synthase Heme Domain with d-Nitroarginine-Containing Dipeptide Inhibitors Bound.

Flinspach, M.,Li, H.,Jamal, J.  et al.
(2004)  Biochemistry  43 : 5181 - 5187

PubMed: 15122883
DOI: 10.1021/bi0361867

The novel binding mode of N-alkyl-N'-hydroxyguanidine to neuronal nitric oxide synthase provides mechanistic insights into NO biosynthesis

Li, H.,Shimizu, H.,Flinspach, M.L.  et al.
(2002)  Biochemistry  41 : 13868 - 13875

DOI: 10.1021/bi020417c

Reaction

Chain : A, B
UniProt : P29476 (NOS1_RAT)
Reaction: EC: Evidence:
Physiological Direction:
2 L-arginine + 3 NADPH + 4 O2 + H(+) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H2O 1.14.13.39 PubMed:15548660, PubMed:1712077
left-to-right PubMed:1712077
Cofactor: Evidence: Note:
heme b ECO:0000250 | UniProtKB:P29475
-
FAD ECO:0000269 | PubMed:11473123
ECO:0000269 | PubMed:15208315
Binds 1 FAD.
FMN ECO:0000269 | PubMed:15208315
Binds 1 FMN.
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin ECO:0000250 | UniProtKB:P29475
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.