Brand  (β version)

PDB ID: 1QX1

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Ligands
Code Name Style Show Link
MPD (4s)-2-methyl-2,4-pentanediol PoSSuM
ZN Zinc ion PoSSuM
FMF 2-deoxy-2-fluoro-beta-D-mannopyranosyl fluoride PoSSuM
Non-standard Residues
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Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
Modification
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Structure summary

Code : 1QX1   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Golgi alpha-mannosidase II D341N mutant complex with 2-F-mannosyl-F
Release Data : 2003-10-07
Compound :
mol_id molecule chains synonym
1 Alpha-mannosidase II A Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, MAN II, Golgi alpha-mannosidase II, AMAN II
ec: 3.2.1.114
fragment: Family 38 catalytic domain (residues 94-1108)
mutation: D341N
Source :
mol_id organism_scientific organism_common expression_system
1 Drosophila melanogaster  (taxid:7227) Fruit fly Drosophila melanogaster  (taxid:7227)
gene: ALPHA-MAN-II OR GMII OR CG18474/CG18802/CG8139
expression_system_common: Fruit fly
expression_system_strain: S2
Authors : Numao, S., Kuntz, D.A., Withers, S.G., Rose, D.R.
Keywords : Glycosyl hydrolase family 38, covalent catalytic intermediate, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.30 Å )
Citation :

Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates.

Numao, S.,Kuntz, D.A.,Withers, S.G.  et al.
(2003)  J.Biol.Chem.  278 : 48074 - 48083

PubMed: 12960159
DOI: 10.1074/jbc.M309249200

Reaction

Chain : A
UniProt : Q24451 (MAN2_DROME)
Reaction: EC: Evidence:
Physiological Direction:
2 H2O + N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha- D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] = 2 alpha-D-mannopyranose + N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- beta-D-GlcNAc}-L-asparaginyl-[protein] 3.2.1.114 PubMed:10504337
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