Brand  (β version)

PDB ID: 1OHJ

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Ligands
Code Name Style Show Link
COP N-(4-carboxy-4-{4-[(2,4-diamino-pteridin-6-ylmethyl)-amino]-benzoylamino}-butyl)-phthalamic acid PoSSuM
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
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Structure summary

Code : 1OHJ   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : HUMAN DIHYDROFOLATE REDUCTASE, MONOCLINIC (P21) CRYSTAL FORM
Release Data : 1998-04-29
Compound :
mol_id molecule chains synonym
1 DIHYDROFOLATE REDUCTASE A DHFR
ec: 1.5.1.3
other_details: COMPLEXED WITH NADPH AND PT523 NA-(4-AMINO-4-DEOXYPTEROYL)-ND-HEMIPHTHALOYL-L-ORNITHINE
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: POTENTIAL
expression_system_strain: JM107
Authors : Cody, V., Galitsky, N., Luft, J.R., Pangborn, W.
Keywords : OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.50 Å )
Citation :

Comparison of two independent crystal structures of human dihydrofolate reductase ternary complexes reduced with nicotinamide adenine dinucleotide phosphate and the very tight-binding inhibitor PT523.

Cody, V.,Galitsky, N.,Luft, J.R.  et al.
(1997)  Biochemistry  36 : 13897 - 13903

PubMed: 9374868
DOI: 10.1021/bi971711l

Methotrexate-Resistant Variants of Human Dihydrofolate Reductase with Substitutions of Leucine 22. Kinetics, Crystallography, and Potential as Selectable Markers

Lewis, W.S.,Cody, V.,Galitsky, N.  et al.
(1995)  J.Biol.Chem.  270 : 5057

Methotrexate-Resistant Variants of Human Dihydrofolate Reductase. Effects of Phe31 Substitutions

Chunduru, S.K.,Cody, V.,Luft, J.R.  et al.
(1994)  J.Biol.Chem.  269 : 9547

Crystal Structure Determination at 2.3 A of Recombinant Human Dihydrofolate Reductase Ternary Complex with Nadph and Methotrexate-Gamma-Tetrazole

Cody, V.,Luft, J.R.,Ciszak, E.  et al.
(1992)  Anti-Cancer Drug Des.  7 : 483

Reaction

Chain : A
UniProt : P00374 (DYR_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 PROSITE- ProRule:PRU00660, PubMed:12096917, PubMed:15039552, PubMed:17569517, PubMed:19196009, PubMed:19478082, PubMed:21876184, PubMed:9719595
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