Brand  (β version)

PDB ID: 1ML3

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Ligands
Code Name Style Show Link
CYX (3-formyl-but-3-enyl)-phosphonic acid PoSSuM
NAD Nicotinamide-adenine-dinucleotide PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1ML3   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Evidences for a flip-flop catalytic mechanism of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase, from its crystal structure in complex with reacted irreversible inhibitor 2-(2-phosphono-ethyl)-acrylic acid 4-nitro-phenyl ester
Release Data : 2003-07-08
Compound :
mol_id molecule chains synonym
1 Glyceraldehyde 3-phosphate dehydrogenase, glycosomal A,B,C,D GAPDH
ec: 1.2.1.12
Source :
mol_id organism_scientific expression_system
1 Trypanosoma cruzi  (taxid:5693) Escherichia coli BL21  (taxid:511693)
gene: gapdh
expression_system_strain: BL21
expression_system_vector_type: pET3a
Authors : Castilho, M.S., Pavao, F., Oliva, G.
Keywords : protein covalent-inhibitor complex, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.5 Å )
Citation :

Evidence for the Two Phosphate Binding Sites of an Analogue of the Thioacyl Intermediate for the Trypanosoma cruzi Glyceraldehyde-3-phosphate Dehydrogenase-Catalyzed Reaction, from Its Crystal Structure.

Castilho, M.S.,Pavao, F.,Oliva, G.  et al.
(2003)  Biochemistry  42 : 7143 - 7151

PubMed: 12795610
DOI: 10.1021/bi0206107

Reaction

Chain : A, B, C, D
UniProt : P22513 (G3PG_TRYCR)
Reaction: EC: Evidence:
Physiological Direction:
D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3- phospho-glyceroyl phosphate + H(+) + NADH 1.2.1.12 PROSITE-ProRule:PRU10009
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