Brand  (β version)

PDB ID: 1M78

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Ligands
Code Name Style Show Link
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
CLZ 5-chloryl-2,4,6-quinazolinetriamine PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1M78   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : CANDIDA ALBICANS DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (NADPH) AND 5-CHLORYL-2,4,6-QUINAZOLINETRIAMINE (GW1225)
Release Data : 2003-03-04
Compound :
mol_id molecule chains synonym
1 DIHYDROFOLATE REDUCTASE A,B DHFR
ec: 1.5.1.3
Source :
mol_id organism_scientific expression_system
1 Candida albicans  (taxid:5476) Escherichia coli BL21(DE3)  (taxid:469008)
gene: DFR1
expression_system_strain: BL21(DE3)
expression_system_cellular_location: cytoplasm
expression_system_vector_type: PLASMID
expression_system_plasmid: P1869
Authors : Whitlow, M., Howard, A.J., Kuyper, L.F.
Keywords : OXIDOREDUCTASE, ANTIFUNGAL TARGET, REDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.71 Å )
Citation :

X-Ray Crystallographic Studies of Candida Albicans Dihydrofolate Reductase. High Resolution Structures of the Holoenzyme and an Inhibited Ternary Complex.

Whitlow, M.,Howard, A.J.,Stewart, D.  et al.
(1997)  J.Biol.Chem.  272 : 30289 - 30298

PubMed: 9374515
DOI: 10.1074/jbc.272.48.30289

X-Ray Crystal Structures of Candida Albicans Dihydrofolate Reductase: High Resolution Ternary Complexes in which the Dihydronicotinamide Moiety of Nadph is Displaced by an Inhibitor

Whitlow, M.,Howard, A.J.,Stewart, D.  et al.
(2001)  J.Med.Chem.  44 : 2928 - 2932

DOI: 10.1021/jm0101444

Selective Inhibitors of Candida Albicans Dihydrofolate Reductase: Activity and Selectivity of 5-(Arylthio)-2,4-Diaminoquinazolines

Chan, J.H.,Hong, J.S.,Kuyper, L.F.  et al.
(1995)  J.Med.Chem.  38 : 3608 - 3616

Characterization of Candida Albicans Dihydrofolate Reductase

Baccanari, D.P.,Tansik, R.L.,Joyner, S.S.  et al.
(1989)  J.Biol.Chem.  264 : 1100 - 1107

Reaction

Chain : A, B
UniProt : P22906 (DYR_CANAX)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 PROSITE- ProRule:PRU00660
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