Brand  (β version)

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centroid:
interaction residue:

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Ligands
Code Name Link
FTP 3-(4-amino-2-trifluoromethyl-pyrimidin-5-ylmethyl)-4-methyl-5-(2-phosphonatooxy-ethyl)-thiazol-3-ium
MG Magnesium ion
POP Pyrophosphate 2-
Code : 1G4T
Header : TRANSFERASE
Title : THIAMIN PHOSPHATE SYNTHASE
Release Data : 2001-09-26
Compound :
mol_id molecule chains
1 THIAMIN PHOSPHATE SYNTHASE A,B
ec: 2.5.1.3
other_details: COMPLEXED WITH TRIFLUORO-THIAMIN PHOSPHATE
Source :
mol_id organism_scientific expression_system
1 Bacillus subtilis  (taxid:1423) Escherichia coli  (taxid:562)
gene: THIC
expression_system_strain: SG13009 WITH PREP4
expression_system_vector_type: PLASMID
expression_system_plasmid: PYZC6927
Authors : Peapus, D.H., Chiu, H.-J., Campobasso, N., Reddick, J.J., Begley, T.P., Ealick, S.E.
Keywords : THIAMIN BIOSYNTHESIS, TIM BARREL, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.55 Å )
Citation :

Structural characterization of the enzyme-substrate, enzyme-intermediate, and enzyme-product complexes of thiamin phosphate synthase.

Peapus, D.H.,Chiu, H.J.,Campobasso, N.  et al.
(2001)  Biochemistry  40 : 10103 - 10114

PubMed: 11513589
DOI: 10.1021/bi0104726

Mechanistic Studies on Thiamin Phosphate Synthase: Evidence for a Dissociative Mechanism

Reddick, J.J.,Nicewonger, R.,Begley, T.P.
(2001)  Biochemistry  40 : 10095 - 10102

DOI: 10.1021/bi010267q

Crystal Structure of Thiamine Phosphate Synthase from Bacillus subtilis at 1.25A Resolution

Chiu, H.-J.,Reddick, J.J.,Begley, T.P.  et al.
(1999)  Biochemistry  38 : 6460 - 6470

DOI: 10.1021/bi982903z

CHARACTERIZATION OF THE BACILLUS SUBTILIS THIC OPERON INVOLVED IN THIAMINE BIOSYNTHESIS

ZHANG, Y.,TAYLOR, S.V.,CHIU, H.-J.  et al.
(1997)  J.BACTERIOL.  179 : 3030 - 3035

Chain : A, B
UniProt : P39594 (THIE_BACSU)
Reaction : 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2).
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO(2).
4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.