Brand  (β version)

PDB ID: 1G42

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Ligands
Code Name Style Show Link
ACT Acetate ion PoSSuM
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
CP2 1,2-dichloro-propane PoSSuM
Non-standard Residues
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2MR N3, n4-dimethylarginine
Glycosylation
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Structure summary

Code : 1G42   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : STRUCTURE OF 1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE HYDROLASE (LINB) FROM SPHINGOMONAS PAUCIMOBILIS COMPLEXED WITH 1,2-DICHLOROPROPANE
Release Data : 2001-10-26
Compound :
mol_id molecule chains synonym
1 1,3,4,6-TETRACHLORO-1,4-CYCLOHEXADIENE HYDROLASE A 1,4-TCDN CHLOROHYDROLASE
ec: 3.8.1.-
Source :
mol_id organism_scientific expression_system
1 Sphingomonas paucimobilis  (taxid:13689) Escherichia coli  (taxid:562)
gene: LINB
expression_system_strain: HB101
expression_system_vector_type: PLASMID
expression_system_plasmid: PMLBH6
Authors : Oakley, A.J., Prokop, Z., Bohac, M., Kmunicek, J., Jedlicka, T., Monincova, M., Kuta-Smatanova, I., Nagata, Y., Damborsky, J., Wilce, M.C.J.
Keywords : LinB dehalogenase alpha/beta-hydrolase halocarbons, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Exploring the structure and activity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26: evidence for product- and water-mediated inhibition.

Oakley, A.J.,Prokop, Z.,Bohac, M.  et al.
(2002)  Biochemistry  41 : 4847 - 4855

PubMed: 11939779
DOI: 10.1021/bi015734i

Crystal structure of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26

Marek, J.,Vevodova, J.,Smatanova, I.K.  et al.
(2000)  Biochemistry  39 : 14082 - 14086

DOI: 10.1021/bi001539c

Reaction

Chain : A
UniProt : P51698 (LINB_SPHIU)
Reaction: EC: Evidence:
Physiological Direction:
1-haloalkane + H2O = a halide anion + a primary alcohol + H(+) 3.8.1.5 HAMAP-Rule:MF_01231, PubMed:10100638, PubMed:9293022
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(3R,6R)-1,3,4,6-tetrachlorocyclohexa-1,4-diene + 2 H2O = 2,5- dichlorocyclohexa-2,5-dien-1,4-diol + 2 chloride + 2 H(+) - PubMed:7691794
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