Brand  (β version)

PDB ID: 1ZZU

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Ligands
Code Name Style Show Link
ACT Acetate ion PoSSuM
DP1 L-N(omega)-nitroarginine-2,4-L-diaminobutyric amide PoSSuM
GOL Glycerol PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
MTL D-mannitol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1ZZU   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Rat nNOS D597N/M336V double mutant with L-N(omega)-Nitroarginine-2,4-L-Diaminobutyric Amide Bound
Release Data : 2005-12-06
Compound :
mol_id molecule chains synonym
1 Nitric-oxide synthase, brain A,B NOS, type I, Neuronal NOS, N-NOS, nNOS, Constitutive NOS, NC-NOS, BNOS
ec: 1.14.13.39
mutation: M336V, D597N
Source :
mol_id organism_scientific organism_common expression_system
1 Rattus norvegicus  (taxid:10116) Norway rat Escherichia coli BL21(DE3)  (taxid:469008)
gene: Nos1, Bnos
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pCWori
Authors : Li, H., Flinspach, M.L., Igarashi, J., Jamal, J., Yang, W., Gomez-Vidal, J.A., Litzinger, E.A., Silverman, R.B., Poulos, T.L.
Keywords : oxydoreductase, enzyme-inhibtor complex, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 1.900 Å )
Citation :

Exploring the Binding Conformations of Bulkier Dipeptide Amide Inhibitors in Constitutive Nitric Oxide Synthases.

Li, H.,Flinspach, M.L.,Igarashi, J.  et al.
(2005)  Biochemistry  44 : 15222 - 15229

PubMed: 16285725
DOI: 10.1021/bi0513610

Structural Basis for Dipeptide Amide Isoform-Selective Inhibition of Neuronal Nitric Oxide Synthase

Flinspach, M.L.,Li, H.,Jamal, J.  et al.
(2004)  Nat.Struct.Mol.Biol.  11 : 54 - 59

PubMed: 14718923
DOI: 10.1038/nsmb704

Reaction

Chain : A, B
UniProt : P29476 (NOS1_RAT)
Reaction: EC: Evidence:
Physiological Direction:
2 L-arginine + 3 NADPH + 4 O2 + H(+) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H2O 1.14.13.39 PubMed:15548660, PubMed:1712077
left-to-right PubMed:1712077
Cofactor: Evidence: Note:
heme b ECO:0000250 | UniProtKB:P29475
-
FAD ECO:0000269 | PubMed:11473123
ECO:0000269 | PubMed:15208315
Binds 1 FAD.
FMN ECO:0000269 | PubMed:15208315
Binds 1 FMN.
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin ECO:0000250 | UniProtKB:P29475
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.