Brand  (β version)

PDB ID: 1ZTT

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Ligands
Code Name Style Show Link
NT Netropsin PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 1ZTT   PDBj   RCSB PDB   PDBe
Header : Transferase/DNA
Title : Netropsin bound to D(CTTAATTCGAATTAAG) in complex with MMLV RT catalytic fragment
Release Data : 2005-08-30
Compound :
mol_id molecule chains
1 5'-D(*CP*TP*TP*AP*AP*TP*TP*C)-3' B
mol_id molecule chains
2 5'-D(P*GP*AP*AP*TP*TP*AP*AP*G)-3' G
mol_id molecule chains synonym
3 Reverse transcriptase A RT
ec: 2.7.7.49
fragment: RT catalytic fragment
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Moloney murine leukemia virus  (taxid:11801) Escherichia coli  (taxid:562)
gene: POL
Authors : Goodwin, K.D., Long, E.C., Georgiadis, M.M.
Keywords : Netropsin, MMLV-RT, Drug-DNA complex, Protein-DNA complex, Transferase-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

A host-guest approach for determining drug-DNA interactions: an example using netropsin.

Goodwin, K.D.,Long, E.C.,Georgiadis, M.M.
(2005)  Nucleic Acids Res.  33 : 4106 - 4116

PubMed: 16049022
DOI: 10.1093/nar/gki717

Reaction

Chain : A
UniProt : P03355 (POL_MLVMS)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.49 PROSITE-ProRule:PRU00405
-
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 PROSITE-ProRule:PRU00405
-
Endonucleolytic cleavage to 5'-phosphomonoester. 3.1.26.4 PROSITE-ProRule:PRU00408
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | PROSITE-ProRule:PRU00405
The RT polymerase active site binds 2 magnesium ions.
Mg(2+) ECO:0000250 | UniProtKB:Q2F7J3
ECO:0000269 | PubMed:16912289
Binds 2 Mn(2+)/Mg(2+) ions for ribonuclease H (RNase H) activity.
Mg(2+) ECO:0000305
Magnesium ions are required for integrase activity. Binds at least 1, maybe 2 magnesium ions.