Brand  (β version)

PDB ID: 1ZSF

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
0ZS N-{(2r,3s)-3-[(tert-butoxycarbonyl)amino]-2-hydroxy-4-phenylbutyl}-L-phenylalanyl-L-alpha-glutamyl-L-phenylalaninamide PoSSuM
Modified Residues
Code Name Link
CSO S-hydroxycysteine PoSSuM

Structure summary

Code : 1ZSF
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : Crystal Structure of Complex of a Hydroxyethylamine Inhibitor with HIV-1 Protease at 2.0A Resolution
Release Data : 2006-04-25
Compound :
mol_id molecule chains synonym
1 PROTEASE RETROPEPSIN A,B HIV-1 PROTEASE
ec: 3.4.23.16
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus 1  (taxid:11676) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PT7Q10H1N
Authors : Duskova, J., Skalova, T., Dohnalek, J., Petrokova, H., Hasek, J.
Keywords : HIV, PROTEASE, PEPTIDOMIMETIC INHIBITOR, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.98 Å )
Citation :

On the role of the R configuration of the reaction-intermediate isostere in HIV-1 protease-inhibitor binding: X-ray structure at 2.0 A resolution.

Duskova, J.,Dohnalek, J.,Skalova, T.  et al.
(2006)  Acta Crystallogr.,Sect.D  62 : 489 - 497

PubMed: 16627941
DOI: 10.1107/S0907444906006718

Reaction

Chain : A, B
UniProt : P03367 (POL_HV1BR)
Reaction : Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).