Brand  (β version)

PDB ID: 1ZP4

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Ligands
Code Name Style Show Link
C2F 5-methyl-5,6,7,8-tetrahydrofolic acid PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Structure summary

Code : 1ZP4   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Glu28Gln mutant of E. coli Methylenetetrahydrofolate Reductase (oxidized) complex with Methyltetrahydrofolate
Release Data : 2005-08-09
Compound :
mol_id molecule chains
1 5,10-methylenetetrahydrofolate reductase A,B,C
ec: 1.7.99.5
mutation: E28Q
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: metF
expression_system_vector_type: PLASMID
expression_system_plasmid: pET23B
Authors : Pejchal, R., Sargeant, R., Ludwig, M.L.
Keywords : TIM BARREL, METHYLTETRAHYDROFOLATE, FLAVIN, REDUCTASE, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Structures of NADH and CH(3)-H(4)Folate Complexes of Escherichia coli Methylenetetrahydrofolate Reductase Reveal a Spartan Strategy for a Ping-Pong Reaction

Pejchal, R.,Sargeant, R.,Ludwig, M.L.
(2005)  Biochemistry  44 : 11447 - 11457

PubMed: 16114881
DOI: 10.1021/bi050533q

Reaction

Chain : A, B, C
UniProt : P00394 (METF_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH 1.5.1.54 PubMed:11371182, PubMed:19610625, PubMed:9922232, PubMed:14275142
right-to-left PubMed:14275142, PubMed:9922232