Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

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Ligands
Code Name Link
0ZR N-{(2r,3s)-3-[(tert-butoxycarbonyl)amino]-2-hydroxy-4-phenylbutyl}-L-phenylalanyl-L-glutaminyl-L-phenylalaninamide
Code : 1ZLF
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : Crystal structure of a complex of mutant HIV-1 protease (A71V, V82T, I84V) with a hydroxyethylamine peptidomimetic inhibitor
Release Data : 2006-04-25
Compound :
mol_id molecule chains synonym
1 PROTEASE RETROPEPSIN A,B HIV-1 PROTEASE
ec: 3.4.23.16
mutation: A71V, V82T, I84V
Source :
mol_id organism_scientific expression_system
1 Human immunodeficiency virus 1  (taxid:11676) Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET24a
Authors : Skalova, T., Dohnalek, J., Duskova, J., Petrokova, H., Hasek, J.
Keywords : HIV, PROTEASE, PEPTIDOMIMETIC INHIBITOR, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

HIV-1 protease mutations and inhibitor modifications monitored on a series of complexes. Structural basis for the effect of the A71V mutation on the active site

Skalova, T.,Dohnalek, J.,Duskova, J.  et al.
(2006)  J.Med.Chem.  49 : 5777 - 5784

PubMed: 16970402
DOI: 10.1021/jm0605583

Chain : A, B
UniProt : P03367 (POL_HV1BR)
Reaction : Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).